We narrowed to 21,673 results for: pET

1. pET11phiC31poly(A)

   Plasmid

   #18942

   Depositor

   Michele Calos

   Article

   Groth et al Genetics. 2004 Apr . 166(4):1775-82.

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Tags

   GFP and His

   Expression

   Bacterial

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human_TFAM_NoMTS_pET28

   Plasmid

   #34705

   Depositor

   David Chan

   Article

   Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

   Insert

   TFAM (TFAM Human)
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Encodes residues 43–246, corresponding to full-le…

   Promoter

   T7

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28CLY1

   Plasmid

   #21760

   Depositor

   Maarten Merkx

   Article

   Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

   Insert

   ECFP -EYFP linked by flexible one SGGSGG repeats
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b_PstS_2

   Plasmid

   #78199

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,A197C)
   

   Expression

   Bacterial

   Mutation

   A17C; A197C (please see depositor comment below)

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET32bplus_trxAChEwt

    Plasmid

    #83916

    Purpose

    Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

    Depositor

    Sarel Fleishman

    Article

    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

    Insert

    Short form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    

    Tags

    His, S-tag, and TRX

    Expression

    Bacterial

    Mutation

    codon optimised for E.coli expression

    Promoter

    T7 promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_BGL3

    Plasmid

    #101912

    Purpose

    Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.

    Depositor

    Steve Shih

    Article

    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

    Insert

    β-glucosidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7+LacO

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22B_SA_PcrA

    Plasmid

    #102999

    Purpose

    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

    Depositor

    Martin Webb

    Insert

    SA_PcrA
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    PcrA 199-2423

    Promoter

    T7

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28CLY3

    Plasmid

    #21762

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pETMutS

    Plasmid

    #13245

    Depositor

    Peggy Hsieh

    Article

    Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

    Insert

    MutS
    

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET28CLY9

    Plasmid

    #21769

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pET28CLY2

    Plasmid

    #21761

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing two SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pET3aTr

    Plasmid

    #64008

    Purpose

    Single donor or transfer plasmid for source of translation cassette for plasmid suite

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGreen_GUS_competitor

    Plasmid

    #55208

    Purpose

    GUS-based microRNA target competitor used for testing function as target mimicry

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Insert

    beta-glucuronidase
    

    Use

    Plant expression

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23a_mod_HuD_FL

    Plasmid

    #65751

    Purpose

    plasmid for bacterial overexpression of HuD

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    6X His and FLAG HA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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