We narrowed to 9,929 results for: tet

1. pTER RB1shRNA human

   Plasmid

   #66886

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

   Depositor

   Maria Alvarado-Kristensson

   Article

   Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

   Insert

   retinoblastoma 1 (RB1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 Tet

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

   Plasmid

   #176027

   Purpose

   construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   rtTA3
   Luc2
   mtagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO Myc ArB WT RNAiR

   Plasmid

   #59806

   Purpose

   Allows the integration of Myc ArB in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora B (AURKB Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   RNAi resistant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO Myc ArC Long WT

   Plasmid

   #59809

   Purpose

   Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora C (AURKC Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTER E2F2shRNA human

   Plasmid

   #66884

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 2 (E2F2 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 Tet

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. CIAR_pcDNA5/FRT/TO

   Plasmid

   #86502

   Purpose

   Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CIAR (SOS1 Human)
   

   Use

   Frt

   Tags

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV (tet operator)

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE-HA-CtIP-siR-N289A-H290A

   Plasmid

   #82032

   Purpose

   Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCWXPGR-pTF-DVL2deltaDIX

   Plasmid

   #114276

   Purpose

   TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   DVL2 (Dvl2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of DIX domain

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN-G1TGt

   Plasmid

   #44508

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   tetR::yEGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pTER E2F3shRNA human

    Plasmid

    #66885

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 3 (E2F3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid

    #82031

    Purpose

    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Non phosphorylatable mutations on 3 of the main A…

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDN-G1TCbt

    Plasmid

    #44515

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRevTRE SGFP2 Inpp5E WT shRNA resistant

    Plasmid

    #161997

    Purpose

    Tet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5E (INPP5E Human)
    

    Use

    Retroviral

    Tags

    SGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 TO FRT Myc ArB K106R

    Plasmid

    #59807

    Purpose

    Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K106R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

    Plasmid

    #139482

    Purpose

    Expresses PhyB621-mCherry-HRasCT in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    PhyB621 (PHYB Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mCherry-HRasCT

    Expression

    Mammalian

    Mutation

    G229L and deletion of aa Y at position 235 in mch…

    Promoter

    Tet responsible element

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK-Venus-TmiR-G13

    Plasmid

    #25741

    Purpose

    Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid

    #112712

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    EGFP
    

    Use

    Tags

    HIV Rev nuclear localization signal (NES), biotin…

    Expression

    Mammalian

    Mutation

    Nucleotide sequence optimized for synthetic gene …

    Promoter

    CMV with Tet repressor binding sites

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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