We narrowed to 21,508 results for: pET

1. pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

   Plasmid

   #241012

   Purpose

   VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

   Depositor

   Cole DeForest

   Article

   Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

   Insert

   GSS-6xHis-SUMO-VidC-bla-FLAG
   

   Tags

   FLAG-tag, His-tag, and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

   Plasmid

   #241011

   Purpose

   VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

   Depositor

   Cole DeForest

   Article

   Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

   Insert

   GSS-6xHis-SUMO-VidC-EGF-FLAG
   

   Tags

   FLAG-tag, His-tag, and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR82: pET-SUMO-USP21 (S93A/S113A/S115A)

   Plasmid

   #240190

   Purpose

   Bacterial expression for USP21 (S93A/S113A/S115A)

   Depositor

   Cynthia Wolberger

   Insert

   USP21 (USP21 Human)
   

   Tags

   6x-His-SUMO

   Expression

   Bacterial

   Mutation

   Changed Serine 93, 113, and 115 to Alanine

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

   Plasmid

   #223790

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   WIPI2d IDR (WIPI2 Human)
   

   Tags

   6xHis-TEV-mCherry

   Expression

   Bacterial

   Mutation

   aa 364-425 only

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

   Plasmid

   #223762

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 IDR (ATG13 Human)
   

   Tags

   6xHis-TEV-mCherry

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

   Plasmid

   #223738

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   NIX (BNIP3L) soluble part (BNIP3L Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   W36A/L39A; deletion of LIR

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)

   Plasmid

   #223739

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   FUNDC1 soluble part, mutant (FUNDC1 Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   Y18A/L21A; deletion of LIR

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)

   Plasmid

   #223749

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with W276A/I279A; deletion of…

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

   Plasmid

   #223724

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   FIP200-CTR (RB1CC1 Human)
   

   Tags

   6xHis-TEV-EGFP

   Expression

   Bacterial

   Mutation

   aa 1429-1591 only

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

    Plasmid

    #229223

    Purpose

    For expression of T. elongatus KaiB protein mutant

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB T. elongatus)
    

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    1-94 (truncated), Y8A, Y94A

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

    Plasmid

    #226349

    Purpose

    Expression of Titin V15P substrate with GS tail

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid

    #169135

    Purpose

    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Coh7
    

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid

    #208626

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 191~307, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid

    #208629

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 212~285, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM-60-NcQDE2 _506-938-del787-838-GGSG_O

    Plasmid

    #147085

    Purpose

    Bacterial Expression of NcQde-2_506-938-del787-838

    Depositor

    Elisa Izaurralde

    Insert

    NcQde-2_506-938-del787-838
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM-60-NcQDE2 _506-938-del787-838-GSG_O

    Plasmid

    #147086

    Purpose

    Bacterial Expression of NcQde-2_506-938-del787-838

    Depositor

    Elisa Izaurralde

    Insert

    NcQde-2_506-938-del787-838
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid

    #170788

    Purpose

    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Mortierella verticillate PRL
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid

    #170789

    Purpose

    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Toxoplasma gondii PRL
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid

    #166422

    Purpose

    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Plasmodium falciparum PRL
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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