We narrowed to 8,514 results for: sgrna

1. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-Nme2_tracr

   Plasmid

   #192146

   Purpose

   Expresses Nme2-sgRNA cloned with BspQI

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nme2 sgRNA scaffold
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZR064_CROPseq-sgCD4-CRISPRi

   Plasmid

   #180270

   Purpose

   Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   CD4 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG_GI2

   Plasmid

   #111593

   Purpose

   mU6-sgRNA GI Library vector

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG_GI3

   Plasmid

   #111594

   Purpose

   hU6-sgRNA GI Library vector

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJZC523

   Plasmid

   #62320

   Purpose

   sgRNA for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

   Plasmid

   #172224

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_ATP1A1_G7

   Plasmid

   #173204

   Purpose

   Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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