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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,001 results for: ef1;

Showing: 4381 - 4400 of 9001 results

  2. pAS133

    Plasmid
    #211504
    Purpose
    AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mTagBF2
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211504-plasmid-map-sequence-id-417794

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  3. pAS134

    Plasmid
    #211505
    Purpose
    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    Venus
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211505-plasmid-map-sequence-id-417792

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  4. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211503-plasmid-map-sequence-id-417791

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  5. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204978-plasmid-map-sequence-id-415579

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  8. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

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  11. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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  13. Fireworks RFP[PTC-] (AVA2515)

    Plasmid
    #85443
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85443-plasmid-map-sequence-id-171526

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Showing: 4381 - 4400 of 9001 results