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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4381 - 4400 of 6962 results

  3. nSaCas9 I744 miniABEMax V82G

    Plasmid
    #135379
    Purpose
    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSaCas9 I744 miniABEMax V82G
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    (V82G)
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135379-plasmid-map-sequence-id-267815

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  4. pcDNA3-sACE2v2.4-sfGFP

    Plasmid
    #149666
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    149666-plasmid-map-sequence-id-298415

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  5. pcDNA3-sACE2v1-sfGFP

    Plasmid
    #145172
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 1
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    145172-plasmid-map-sequence-id-292576

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  7. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    131124-plasmid-map-sequence-id-257454

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  9. CaMKIV-dCT

    Plasmid
    #126422
    Purpose
    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Publication
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126422-plasmid-map-sequence-id-249664

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  12. eGFP-proα2(I)-L

    Plasmid
    #120160
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Publication
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120160-plasmid-map-sequence-id-233870

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  13. Opto-D2R

    Plasmid
    #106068
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)
    Depositor
    Harald Janovjak
    Publication
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Opto-D2R
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106068-plasmid-map-sequence-id-232402

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  14. GFP-LiMETER-v1

    Plasmid
    #113933
    Purpose
    Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP
    Depositor
    Yubin Zhou
    Publication
    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV2 and Rit polybasic domain (PB)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113933-plasmid-map-sequence-id-225305

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Showing: 4381 - 4400 of 6962 results