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Optical functionalization of human Class A orphan G-protein-coupled receptors.
Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, Hirschfeld PP, Schwarz J, Janovjak H
Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
PubMed Article

G-protein-coupled receptors (GPCRs) comprise the largest human membrane receptor family, relay environmental stimuli to essential changes in cell behavior and represent prime drug targets. This collection of GPCR expression plasmids for optogenetic experiments consists of >60 chimeric receptors that contain the signaling domains of human orphan and understudied GPCRs functionally linked to the light-sensing GPCR rhodopsin. The replacement of ligand-binding sites with rhodopsin permits activating cellular signaling pathways using light in the absence of the (unknown) chemical ligands. The following human receptor genes are included in the collection: GPR1, GPR3, GPR4, GPR6, GPR12, GPR15, GPR17, GPR18, GPR19, GPR20, GPR21, GPR22, GPR25, GPR26, GPR27, GPR31, GPR32, GPR33, GPR34, GPR35, GPR37, GPR37L1, GPR39, GPR42, GPR45, GPR50, GPR52, GPR55, GPR61, GPR62, GPR63, GPR65, GPR68, GPR75, GPR78, GPR82, GPR83, GPR84, GPR85, GPR87, GPR88, GPR119, GPR120, GPR132, GPR135, GPR139, GPR141, GPR142, GPR146, GPR148, GPR149, GPR150, GPR151, GPR152, GPR153, GPR160, GPR161, GPR162, GPR171, GPR173, GPR174, GPR176, GPR182 and GPR183.

Additionally, this plasmid collection contains chimeric control receptors that are light-activated variants of nine prototypical GPCRs and were generated using the same methodology: Adrenergic receptors beta2-AR and alpha1-AR, dopamine receptors D1R and D2R, muscarinic acetylcholine receptors M1R, M2R and M3R, adenosine receptor A2A and free fatty acid receptor FFR3. Collectively, these plasmids provide access to an optically controlled protein library for exploring the physiology and therapeutic potential of GPCRs.

Janovjak_Lab_GPCRs

Visit the Janovjak Lab website for additional useful information.

Plasmids from Article

ID Plasmid Purpose  
105999Opto-GPR1Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR1; Opto-GPR1; A1)
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106000Opto-GPR3Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)
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106001Opto-GPR4Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)
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106002Opto-GPR6Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR6; Opto-GPR6; A4)
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106003Opto-GPR12Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR12; Opto-GPR12; A5)
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106004Opto-GPR15Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR15; Opto-GPR15; A6)
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106005Opto-GPR17Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR17; Opto-GPR17; A7)
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106007Opto-GPR19Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)
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106008Opto-GPR20Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR20; Opto-GPR20; A10)
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106009Opto-GPR21Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR21; Opto-GPR21; A11)
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106010Opto-GPR22Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR22; Opto-GPR22; A12)
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106012Opto-GPR26Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR26; Opto-GPR26; B2)
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106013Opto-GPR27Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)
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106014Opto-GPR31Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)
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106015Opto-GPR32Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR32; Opto-GPR32; B5)
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106016Opto-GPR33Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR33; Opto-GPR33; B6)
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106017Opto-GPR34Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR34; Opto-GPR34; B7)
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106018Opto-GPR35Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR35; Opto-GPR35; B8)
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106019Opto-GPR37Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37; Opto-GPR37; B9)
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106020Opto-GPR37L1Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)
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106021Opto-GPR39Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR39; Opto-GPR39; B11)
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106022Opto-GPR42Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)
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106023Opto-GPR45Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR45; Opto-GPR45; C1)
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106024Opto-GPR50Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR50; Opto-GPR50; C2)
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106025Opto-GPR52Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR52; Opto-GPR52; C3)
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106026Opto-GPR55Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)
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106027Opto-GPR61Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)
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106029Opto-GPR63Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR63; Opto-GPR63; C7)
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106030Opto-GPR65Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR65; Opto-GPR65; C8)
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106031Opto-GPR68Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR68; Opto-GPR68; C9)
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106032Opto-GPR75Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)
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106033Opto-GPR78Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR78; Opto-GPR78; C11)
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106034Opto-GPR82Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR82; Opto-GPR82; C12)
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106035Opto-GPR83Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR83; Opto-GPR83; D1)
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106037Opto-GPR85Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR85; Opto-GPR85; D3)
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106038Opto-GPR87Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR87; Opto-GPR87; D4)
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106039Opto-GPR88Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR88; Opto-GPR88; D5)
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106040Opto-GPR119Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)
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106041Opto-GPR120Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR120; Opto-GPR120; D7)
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106042Opto-GPR132Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR132; Opto-GPR132; D8)
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106043Opto-GPR135Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR135; Opto-GPR135; D9)
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106044Opto-GPR139Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)
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106045Opto-GPR141Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)
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106046Opto-GPR142Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR142; Opto-GPR142; D12)
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106047Opto-GPR146Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)
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106048Opto-GPR148Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR148; Opto-GPR148; E2)
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106049Opto-GPR149Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)
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106050Opto-GPR150Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)
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106051Opto-GPR151Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR151; Opto-GPR151; E5)
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106052Opto-GPR152Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR152; Opto-GPR152; E6)
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106053Opto-GPR153Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR153; Opto-GPR153; E7)
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106054Opto-GPR160Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR160; Opto-GPR160; E8)
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106055Opto-GPR161Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR161; Opto-GPR161; E9)
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106056Opto-GPR162Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR162; Opto-GPR162; E10)
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106057Opto-GPR171Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR171; Opto-GPR171; E11)
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106058Opto-GPR173Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)
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106059Opto-GPR174Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR174; Opto-GPR174; F1)
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106060Opto-GPR176Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)
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106061Opto-GPR182Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR182; Opto-GPR182; F3)
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106062Opto-GPR183Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR183; Opto-GPR183; F4)
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106063Opto-beta2ARExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)
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106064Opto-alpha1ARExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)
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106065Opto-FFR3Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-FFR3; Opto-FFR3; F7; 718)
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106066Opto-A2ARExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)
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106067Opto-D1RExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-D1R; Opto-D1R; F9; 720)
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106068Opto-D2RExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)
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106069Opto-M1RExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)
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106070Opto-M2RExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)
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106071Opto-M3RExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M3R; Opto-M3R; H1; 724)
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