We narrowed to 24,896 results for: Nes;

1. pRSET a sfMatryoshCaMP6s

   Plasmid

   #100020

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfMatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB3040(XII-4 MarkerFree)

   Plasmid

   #73280

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLacIBD::SDRX (GB0673)

   Plasmid

   #68183

   Purpose

   Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   LacI Binding Domain fused to SRDX Transcriptional Repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CSK21_HUMAN_D0

   Plasmid

   #79707

   Purpose

   This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK21
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71345

   Purpose

   Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   hOTC_Cyan Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. GAG-PKB-pcDNA3

   Plasmid

   #24651

   Depositor

   Jim Woodgett

   Article

   Jones et al J Exp Med. 2000 May 15. 191(10):1721-34.

   Insert

   PKB (AKT1 Human)
   

   Use

   Tags

   GAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCfB3037(XI-5 MarkerFree)

   Plasmid

   #73277

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

   Plasmid

   #101213

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K855A

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PatoG-OG241

    Plasmid

    #72839

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PatoG

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. KAPCA_HUMAN_D0

    Plasmid

    #79741

    Purpose

    This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KAPCA
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. KIF5C(1-560)-EF(C)

    Plasmid

    #61675

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-EF(C) (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. KIF5C(1-560)-IA

    Plasmid

    #61671

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to IA linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-IA (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDS2

    Plasmid

    #239459

    Purpose

    Simultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusion

    Depositor

    Miguel de Lucas

    Article

    Banda et al Sci. Adv., 11, eadw9153 (2025)

    Type

    Empty backbone

    Use

    Tags

    3xHA:VENUS:2A:mTurqN7

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-MFSD6-Puro

    Plasmid

    #239953

    Purpose

    Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo

    Plasmid

    #239947

    Purpose

    Expression of MFSD8 loop 9 tagged with rabbit IgG Fc2

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    Fc2-MFSD8(L9)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-MFSD8-Puro

    Plasmid

    #239954

    Purpose

    Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD8
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF_AaRaf1/Raf2/BSD2

    Plasmid

    #229515

    Purpose

    Expresses Anthoceros agrestis chaperones: Raf1,Raf2,BSD2

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1
    Rubisco accumulation factor 2
    BSD2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. UBQ10pro-nlsABACUS2-400n-NosT

    Plasmid

    #203728

    Purpose

    Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10pro 1500bp

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2371

    Plasmid

    #225637

    Purpose

    High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

    Insert

    Ple388 (PITX3 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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