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We narrowed to 6,508 results for: nls

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Showing: 4401 - 4420 of 6508 results

  2. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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  5. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  9. pMB63

    Plasmid
    #47945
    Purpose
    Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    eef-1A.1 (eft-3)
    Available Since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    47945-plasmid-map-sequence-id-68805

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  12. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  13. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid
    #92293
    Purpose
    Expression of humanized Mb3Cpf1
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92293-plasmid-map-sequence-id-181495

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  15. mCerulean-Nucleus-12

    Plasmid
    #54556
    Purpose
    Localization: Nucleus, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCerulean-NLS
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    54556-plasmid-map-sequence-id-492859

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  17. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  20. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

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Showing: 4401 - 4420 of 6508 results