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We narrowed to 18,824 results for: nls

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Showing: 4401 - 4420 of 18824 results

  3. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Publication
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  4. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid
    #188682
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188682-plasmid-map-sequence-id-375035

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  5. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid
    #188681
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188681-plasmid-map-sequence-id-375018

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  6. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid
    #188683
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188683-plasmid-map-sequence-id-375016

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  7. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161817-plasmid-map-sequence-id-320651

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  8. pAAV_BiVIPe4_dTomato_nlsdTomato (AAV PHP.eB)

    Viral prep
    #213855-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiVIPe4_dTomato_nlsdTomato (#213855). In addition to the viral particles, you will also receive purified pAAV_BiVIPe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the VIP interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    dTomato
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only

  9. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

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  14. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid
    #226114
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226114-plasmid-map-sequence-id-448323

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  15. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211844
    Purpose
    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.
    Depositor
    Marcela Rojas-Pierce
    Publication
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Insert
    LRR-NEL
    Use
    Synthetic Biology
    Tags
    3xNLS-HA and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    UBIQUITIN10
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    211844-plasmid-map-sequence-id-416933

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  17. Golden GATEway Cloning Kit

    Plasmid kit
    #1000000054
    Purpose
    A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.
    Depositor
    Joachim Wittbrodt
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish Expression
    Cloning type
    Golden Gate
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

    Plasmid kit
    #1000000154
    Purpose
    Targeted genome integration in human cells, zebrafish embryos, and pig fibroblasts.
    Depositor
    Jeffrey Essner
    Application
    Genome Editing
    Vector type
    Mammalian Expression, Zebrafish Expression
    Editing type
    CRISPR
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV_BiCHATe27_dTomato_nlsdTomato (AAV PHP.eB)

    Viral prep
    #213829-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_dTomato_nlsdTomato (#213829). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    dTomato
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. pMSCVpuro-F-Gcn5-NLSm

    Plasmid
    #63708
    Purpose
    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells
    Depositor
    Kai Ge
    Publication
    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:
    Insert
    Gcn5 (Kat2a Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    mutated K425, K427 and K438 to R (arginine)
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4401 - 4420 of 18824 results