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pRS313 - prp8 V1862Y

Plasmid

#90972

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1862Y

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 H1863E

Plasmid

#90973

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

H1863E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 K1864E

Plasmid

#90974

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

K1864E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 N1869D

Plasmid

#90975

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

N1869D

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1870N

Plasmid

#90976

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1870N

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 I1875T

Plasmid

#90977

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

I1875T

Availability

Academic Institutions and Nonprofits only

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pMSCVFLAG-ZFP423

Plasmid

#24764

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
pINDUCER20_mStrawberry

Plasmid

#161734

Purpose

Doxycycline-inducible expression of mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

mStrawberry

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCART0016_CROPseq-CAR-Puro_GPC3-BBz

Plasmid

#242985

Purpose

GPC3-BBz CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GPC3-BBz CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pC1 Grx1-roCherry-mito

Plasmid

#141069

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Tags

dMTS

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threoni…

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Amber-free pNL4-3 FLuc Env-RT-

Plasmid

#238415

Purpose

An amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene, RT and Env impaired.

Depositor

Maolin Lu

Article

Xu et al STAR Protoc. 2025 Jul 26;6(3):103995. do

Insert

Firefly luciferase (LOC116160065 Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Alternation of amber stop codons "TAG" …

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11-mCherry

Plasmid

#179682

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(31-114)

Plasmid

#99514

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(31-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Mutation

internal inversion of nucleotides 265-312 of the …

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEVOL_PylRS(AF)-tRNA

Plasmid

#223511

Purpose

PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Inserts

Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
tRNA Pyl

Tags

6xHis

Expression

Bacterial

Mutation

Y306A; Y384F

Promoter

araBAD, rrnB, and glnS and proK

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-SypHer3s-dmito

Plasmid

#108119

Purpose

Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

Depositor

Vsevolod Belousov

Article

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s-dmito

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEd

Plasmid

#193845

Purpose

Express TadCBEd (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCART0017_CROPseq-CAR-Puro_GD2-BBz

Plasmid

#242984

Purpose

GD2-BBz 14g2a CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GD2-BBz 14g2a CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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