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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 68,994 results for: ang

Showing: 11201 - 11250 of 68994 results

  6. pYPQ265E9

    Plasmid
    #213472
    Purpose
    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tadde-32aa linker-zspc…
    Tags
    NO
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213472-plasmid-map-sequence-id-426728

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  8. AXBE

    Plasmid
    #204605
    Purpose
    Mammalian expression of adenine transversion base editor AXBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204605-plasmid-map-sequence-id-404718

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  16. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62204-plasmid-map-sequence-id-101817

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  19. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid
    #213965
    Purpose
    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
    Depositor
    Wensheng Wei
    Article
    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213965-plasmid-map-sequence-id-422887

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  23. pBT3024

    Plasmid
    #122542
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_mt::CemOrange2
    Use
    Tags
    MLSLRQSIRFFKPATRTLCSSRTLL
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122542-plasmid-map-sequence-id-238125

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  27. pCMV-MtAcKRS (human-opti)

    Plasmid
    #164195
    Purpose
    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
    Depositor
    Tao Liu
    Article
    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2
    Insert
    MtAcKRS-478(human-opti)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    D76G; L325M; L329I; Y330F; L333A; C372F
    Promoter
    CMV
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164195-plasmid-map-sequence-id-325763

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  30. pAC84-pCR8-dCas9

    Plasmid
    #48218
    Purpose
    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A)
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48218-plasmid-map-sequence-id-69495

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  37. pDest27-mBIN1+13

    Plasmid
    #61087
    Purpose
    Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    mouse BIN1 with exon 13, excluding exon 7, 11, 14-17
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. pDest27-mBIN1

    Plasmid
    #61086
    Purpose
    Express N-terminal GST tagged-mouse BIN1 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    smallest constitutive BIN1excluding exon 7, 11, 13-17
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  41. pBT3019

    Plasmid
    #122541
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::SKL
    Use
    Tags
    SKL
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122541-plasmid-map-sequence-id-238135

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  42. pDest27-mBIN1+17

    Plasmid
    #61096
    Purpose
    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 11201 - 11250 of 68994 results