We narrowed to 29,478 results for: SCR

1. Sema3a(L, del13)-AP-His

   Plasmid

   #72011

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT-MCS-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80058

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ncam1_1.18.1-AP-His

   Plasmid

   #71962

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_1.18.1 (Ncam1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Ncam1_0.0.0-AP-His

   Plasmid

   #71960

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_0.0.0 (Ncam1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362

   Plasmid

   #31355

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   Brd4 1224-1362 (BRD4 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGLB2 5'3'

   Plasmid

   #49346

   Purpose

   luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

   Depositor

   Melissa Rogers

   Article

   Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

   Inserts

   Bmp2 distal promoter (Bmp2 Mouse)
   Bmp2 3' UTR (Bmp2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   -

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLCKO_EIF3D_sgRNA_1

   Plasmid

   #74187

   Purpose

   lentiviral vector expressing sgRNA targeting EIF3D

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   EIF3D sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWay21-MKP-1 ASA

   Plasmid

   #13479

   Depositor

   Anton Bennett

   Article

   Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

   Insert

   MKP-1ASA (Dusp1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Arginine 53 to Alanine, Changed Arginine…

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-HsSENP6

   Plasmid

   #71469

   Purpose

   Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 6 (SENP6 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Sema7a-AP-His

    Plasmid

    #72049

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCKO_EIF3D_sgRNA_4

    Plasmid

    #74188

    Purpose

    lentiviral vector expressing sgRNA targeting EIF3D

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    EIF3D sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. shYES1 # 2

    Plasmid

    #42547

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

13. shYES1 # 1

    Plasmid

    #42546

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

14. Plxna1-AP-His

    Plasmid

    #71996

    Purpose

    Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna1 (Plxna1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80056

    Purpose

    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    flk1

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTSlb-Q758C

    Plasmid

    #60729

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2-mqgDUX4mqg*

    Plasmid

    #21177

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 6163 (numbering acco…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pJZC41

    Plasmid

    #62332

    Purpose

    sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    PCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 375 pME-fli1a

    Plasmid

    #49001

    Purpose

    full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    friend leukemia integration 1a (fli1 Zebrafish)
    

    Use

    Gateway middle entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAP4K3-GFP K45E

    Plasmid

    #21734

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of the ATP-binding site (K45E). This ver…

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

21. TREML1-bio-His

    Plasmid

    #51857

    Purpose

    Expresses full-length Trem-like transcript 1 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TREML1 (TREML1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFPN1-N-gamma-tubulin (1-333)

    Plasmid

    #87858

    Purpose

    Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Fragment from aa 1 to 333

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid

    #67666

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1Nmut*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Sema4c-AP-His

    Plasmid

    #72030

    Purpose

    Expresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4c (Sema4c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YES1 (T348I) in pLX303

    Plasmid

    #42564

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T348I

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

26. KHBD00482

    Plasmid

    #39590

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12287 (pdm2 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

27. Dscam1_7.27.30 EC10-Fc-His

    Plasmid

    #72190

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.30
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 496 pSG5L HA RB del651 (NAAIRS)

    Plasmid

    #10726

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del651 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 651 to 656 were replaced by NAAIRS, a fl…

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

29. 500 pSG5L HA RB del685 (NAAIRS)

    Plasmid

    #10729

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del685 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 685 to 690 were replaced by NAAIRS, a fl…

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

30. Sema6a.a-Fc-His

    Plasmid

    #72163

    Purpose

    Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6a.a (Sema6a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Sema3f(L,+)-Fc-His

    Plasmid

    #72147

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Sema6b(L)-Fc-His

    Plasmid

    #72165

    Purpose

    Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6b (Sema6b Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Sema4c-Fc-His

    Plasmid

    #72156

    Purpose

    Expresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4c (Sema4c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. KHBD00642

    Plasmid

    #39706

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5748 (Hsf Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

35. Sema3a(L, del3)-AP-His

    Plasmid

    #72010

    Purpose

    Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3a (Sema3a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ncam1_1.0.1-AP-His

    Plasmid

    #71961

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.0.1 (Ncam1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GAL-p300-1000-1600

    Plasmid

    #89101

    Purpose

    mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1000-1600

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCGN-BCL-6Δ (pCGN-BCL6-delta)

    Plasmid

    #40338

    Depositor

    Alexander Dent

    Article

    Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

    Insert

    BCL-6Δ (BCL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Missing four of six zinc fingers (see note below)…

    Promoter

    CMV

    Available since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

39. Sema3b(L)-AP-His

    Plasmid

    #72013

    Purpose

    Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3b (Sema3b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-sgCCR5

    Plasmid

    #83930

    Purpose

    Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCCR5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge