1. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pcDNA3-P1-N-miRFP670-C

   Plasmid

   #159438

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-mTAZ-WW (86)

   Plasmid

   #40915

   Depositor

   Michael Yaffe

   Article

   Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains mTAZ 14-3-3 binding site and WW domain (…

   Promoter

   Tac

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-mTAZ-WW (43)

   Plasmid

   #40916

   Depositor

   Michael Yaffe

   Article

   Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains mTAZ WW domain (43 amino acids, 122-164)

   Promoter

   Tac

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pOPINF HopF2

   Plasmid

   #228397

   Purpose

   Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   HopF2 (hopF2 Pseudomonas syringae pv. tomato)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR221-HUWE1_R4187C

   Plasmid

   #187141

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   R4187C

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221-HUWE1_F3194S

   Plasmid

   #187139

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   F3194S

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221-HUWE1_H669Q

   Plasmid

   #187137

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   H669Q

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221-HUWE1_H3962D

   Plasmid

   #187130

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   H3962D

   Promoter

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-P1-N-mCherry-C

   Plasmid

   #159437

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   mCherry
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST_FLAG-HUWE1_C4341S

    Plasmid

    #187156

    Purpose

    mammalian cell expression FLAG-HUWE C4341S

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    C4341S

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDARMO_CMVT_FLAG-HUWE1

    Plasmid

    #187121

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hWIP1 FLAG

    Plasmid

    #28105

    Depositor

    Lawrence Donehower

    Article

    Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

    Insert

    WIP1 (PPM1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HUWE1-sgRNA-1

    Plasmid

    #86924

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA1 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

17. HUWE1-sgRNA-2

    Plasmid

    #86925

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA2 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

18. Human HUWE1 (3993-4374)

    Plasmid

    #110754

    Purpose

    Bacterial Expression of HUWE1 HECT domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dead Streptavidin-SpyCatcher

    Plasmid

    #59547

    Purpose

    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead-dloop-Streptavidin-SpyCatcher
    

    Use

    Tags

    N-terminally shortened SpyCatcher

    Expression

    Bacterial

    Mutation

    PolyAsp in L3/4 loop for ion-exchange

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEYFP-C1-hSERT

    Plasmid

    #70103

    Purpose

    mammalian expression of hSERT with N-terminal YFP fusion

    Depositor

    Harald Sitte

    Article

    Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

    Insert

    SERT (SLC6A4 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge