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HP17 sfGFP::P8

Plasmid

#235448

Purpose

Helper plasmid with sfGFP gene inserted at gene VIII locus

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTrcHIS2-MtFAAH1

Plasmid

#243679

Purpose

Expresses Medicago truncatula FAAH1 isoform in E. coli.

Depositor

Kent Chapman

Article

Arias-Gaguancela et al Sci Rep. 2023 May 9;13(1):7486. doi: 10.

Insert

Medicago truncatula Fatty Acid Amide Hydrolase 1

Tags

c-myc, 6xHIS

Expression

Bacterial

Promoter

trc

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTrcHIS2-MtFAAH2a

Plasmid

#243680

Purpose

Expresses Medicago truncatula FAAH2a isoform in E. coli.

Depositor

Kent Chapman

Article

Arias-Gaguancela et al Sci Rep. 2023 May 9;13(1):7486. doi: 10.

Insert

Medicago truncatula Fatty Acid Amide Hydrolase 2a

Tags

c-myc, 6xHIS

Expression

Bacterial

Promoter

trc

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-eGFP

Plasmid

#242758

Purpose

Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-EGFP

Expression

Bacterial

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_UCOE_TRE3VG_mCherry-Responder

Plasmid

#230053

Purpose

It presents the UCOE sequence and the TRE3VG inducible promoter allowing a more stable dox-dependent inducible activation of mCherry though the OPTi-OX platform across differentiation of hiPSCs

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

UCOE

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCLYBL-Neo_TRE3VG_mCherry_Responder

Plasmid

#230054

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of mCherry through the OPTi-OX platform. The CLYBL GSH supports higher transgene expression

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

TRE3VG_mCherry

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-NiVop08-Foldon

Plasmid

#200390

Purpose

Mammalian cell expression of Nipah virus fusion protein with prefusion stabilizing mutations

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiVop08 Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-488), L104C, I114C, L172F, S191P

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJV505

Plasmid

#180078

Purpose

MS.SenLT2II_L85Q expression construct to improve transformation in S. enterica LT2

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.SenLT2II_L85Q

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV303

Plasmid

#180079

Purpose

M.SenLT2I expression construct to improve transformation in S. enterica LT2

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.SenLT2I

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV457

Plasmid

#180080

Purpose

M.BbrUI expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUI

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV461

Plasmid

#180081

Purpose

M.BbrUIII expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUIII

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-454TAG

Plasmid

#240063

Purpose

Express AAV2-KO (R585,588A) capsid with ncAA mutant at site 454

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2-454-TAG, R585,588A

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-454TAG

Plasmid

#240058

Purpose

Express AAV2 capsid with ncAA mutant at site 454

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2 Rep Cap-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (27 kDa)

Plasmid

#231633

Purpose

Mammalian expression of the full-length mouse chaperone protein proSAAS.

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Expression

Mammalian

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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MZB164

Plasmid

#217842

Purpose

10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

HaloTag

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Promoter

T7 Promoter

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sga-Cas9

Plasmid

#227005

Purpose

Mammalian expression of Nuclease active S. gallolyticus Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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GMR11F02-Gal4 UAS-GFP

Plasmid

#230907

Purpose

Tissue-specific expression of GFP in wing and haltere imaginal discs.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Expression

Insect

Promoter

GMR11F02Gal4-UAS

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UAST-FRT-GFP-FRT-uMCas12a+nls1x

Plasmid

#230908

Purpose

Double inducible expression of Cas12a+ with Gal4 and Flp.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

Cas12a+

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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small ubiquitin-like modifier 3 (SUMO3)

Plasmid

#221053

Purpose

Expression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

SUMO 3 (SUMO3 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC1_pPromALB_enh5898_FireflyLuc

Plasmid

#220285

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 5898

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC2_pPromALB_enh29774_FireflyLuc

Plasmid

#220286

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 29774

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC3_pPromALB_enh50363_FireflyLuc

Plasmid

#220287

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50363

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC4_pPromALB_enh50358_FireflyLuc

Plasmid

#220288

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50358

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL17_pPromALB_enhALB_FireflyLuc

Plasmid

#220283

Purpose

Firefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL18_pPromALB_enhALB_RenillaLuc

Plasmid

#220284

Purpose

Renilla luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 (Promega)

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-HsTtyh1-TwinStrep-His

Plasmid

#211332

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Tweety homology protein 1

Use

Lentiviral

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

W417R

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR90

Plasmid

#203914

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

GSC2 plus flanking (GSC2 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR89

Plasmid

#203423

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

EGC3 plus flanking (EGC3 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-nCas9n

Plasmid

#217487

Purpose

Expression of nuclear nCas9n

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_F305A_S306A_H307A

Plasmid

#210793

Purpose

pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F305A_S306A_H307A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pkk223_TtMutY_D147N_E46Q

Plasmid

#210792

Purpose

pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D147N_E46Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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