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Showing: 6991 - 7020 of 14826 results

  1. SMT205

    Plasmid
    #134817
    Purpose
    SMT201 with mutated RBS for DHFR, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134817-plasmid-map-sequence-id-274011

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  9. Gal4pBS34-Pleum

    Plasmid
    #60334
    Purpose
    Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    60334-plasmid-map-sequence-id-96613

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  12. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

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  14. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  18. RFN_EGFP_47

    Plasmid
    #60071
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pOCC207

    Plasmid
    #118869
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mCherry
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118869-plasmid-map-sequence-id-232318

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  26. pGS1011

    Plasmid
    #63783
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Strep-tag II followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63783-plasmid-map-sequence-id-106469

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  27. Gal4pBS12-Pleum

    Plasmid
    #60326
    Purpose
    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    60326-plasmid-map-sequence-id-96590

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Showing: 6991 - 7020 of 14826 results