We narrowed to 24,589 results for: NOL

1. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. gag-MCP-pol

   Plasmid

   #211370

   Purpose

   Packaging plasmid for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-MCP-pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EasyFusion T2A-H2B-GFP

   Plasmid

   #112851

   Purpose

   General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   No

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG1171 FIREmate-ECFP-Giantin

   Plasmid

   #205166

   Purpose

   Expresses FIREmate-ECFP-Giantin in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Giantin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTlpA_mCherry

   Plasmid

   #229146

   Purpose

   Promoter strength evaluation of PtlpA

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   PtlpA

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 ZoAl N-1F

   Plasmid

   #203763

   Purpose

   For expression of N-flag tagged ZoAl R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGWB501NL3F10H

   Plasmid

   #141286

   Purpose

   No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Type

   Empty backbone

   Use

   Luciferase; Gateway

   Tags

   FLAG (x3), HIS (x10), and NLUC

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAMD1-2

   Plasmid

   #61564

   Purpose

   Standardized mini-Tn5 delivery plasmid, kanamycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL2B -1538/+64

    Plasmid

    #24942

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV-U6+27-Tornado-RhoBAST

    Plasmid

    #196341

    Purpose

    Overexpression of circular RhoBAST

    Depositor

    Murat Sunbul

    Article

    Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

    Insert

    Tornado-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge