We narrowed to 32,602 results for: Eng

1. px458_Conc3

   Plasmid

   #134450

   Purpose

   Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICH51288

   Plasmid

   #50269

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-EF1a-V5-LIC

   Plasmid

   #120247

   Purpose

   For LIC cloning with N-terminal V5 tag

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHelper_TetR/pTet-Tnpase (carbR)

   Plasmid

   #213911

   Purpose

   ATc-inducible himar1C9 transposase expression - carb/amp resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMZ4004

   Plasmid

   #204401

   Purpose

   A 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

   Inserts

   mCinR
   GFP
   

   Use

   Synthetic Biology

   Promoter

   PftsH and Synthetic promoter PCin0102D

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSuper-gmiR-122

   Plasmid

   #171554

   Purpose

   a human miR-122-expressing plasmid

   Depositor

   Yan Zeng

   Article

   Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

   Insert

   miR-122 (MIR122 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. px459V2.0_Conc3

   Plasmid

   #134458

   Purpose

   Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRKO

   Plasmid

   #49456

   Purpose

   loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

   Depositor

   Marc Gartenberg

   Article

   Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

   Insert

   loxP-kanMX-loxP cassette
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tol2-krt4-RFP

   Plasmid

   #97149

   Purpose

   As a control of miRNA sponge

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Type

   Empty backbone

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP_sfCherry2(11)_Keratin

    Plasmid

    #82604

    Purpose

    Expresses sfCherry2(11) tagged keratin in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_Keratin
    

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-NLS-BirA-2A-mCherry

    Plasmid

    #127781

    Purpose

    BirA construct in a PCI nls backbone driving mCherry activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    BirA
    

    Use

    Unspecified

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSuper-gmiR-124

    Plasmid

    #171555

    Purpose

    a human miR-124-expressing plasmid

    Depositor

    Yan Zeng

    Article

    Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

    Insert

    miR-124 (MIR124-1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BD2

    Plasmid

    #37486

    Depositor

    Linzhao Cheng

    Article

    Zou et al Blood. 2011 Oct 27;118(17):4599-608. Epu

    Insert

    loxP-PGK-Hyg-loxP
    

    Use

    Ipsc gene targeting

    Promoter

    PGK

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Tags

    3XHA

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IBMc090

    Plasmid

    #161937

    Purpose

    Carries engineered transposon for IBM

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
    

    Expression

    Bacterial

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICH51266

    Plasmid

    #50267

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGF012

    Plasmid

    #48849

    Purpose

    Provides a sulfadiazine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pMAS::SulfadiazineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition sites in SulfR removed …

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK_Sox9_enh_349_Citrine

    Plasmid

    #130594

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox9_enh_349
    

    Use

    Enhancer reporter

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge