We narrowed to 4,802 results for: pcr

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Neurl1-TOPO

Plasmid

#17281

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
UMGIII

Plasmid

#245860

Purpose

Archael (Uncultured marine group III euryarchaeote clone 108exp4) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pToxAmp12

Plasmid

#218286

Purpose

Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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OXGR1-DuET

Plasmid

#213363

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OXGR1 (OXGR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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HCA3-DuET

Plasmid

#213309

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HCA3 (HCAR3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - I438M in pENTR

Plasmid

#216591

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway Cloning

Mutation

I438M

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - R382H in pENTR

Plasmid

#216590

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway Cloning

Mutation

R382H

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G305S in pENTR

Plasmid

#216589

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway Cloning

Mutation

G305S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - D252H in pENTR

Plasmid

#216588

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway Cloning

Mutation

D252H

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - E122K in pENTR

Plasmid

#216587

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway Cloning

Mutation

E122K

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G70S in pENTR

Plasmid

#216586

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway Cloning

Mutation

G70S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/LYCHOS WT-FLAG

Plasmid

#209315

Purpose

Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/FLAG Metap2

Plasmid

#209314

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CRF0.5

Plasmid

#208660

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC-GST-GINIP

Plasmid

#204844

Purpose

Express GST tagged-GINIP in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

PHF24 (KIAA1045 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFB-CG-LYCHOS WT-HA-GFP

Plasmid

#199687

Purpose

Expression of LYCHOS WT in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Tags

10xHis, EGFP, HA, HRV 3C, and His

Expression

Insect

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED WT-FLAG

Plasmid

#199679

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED WT (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTL370

Plasmid

#69880

Purpose

attB flanked genomic Cirl for integration at attP sites.

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pZA7

Plasmid

#158485

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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