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pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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mPlum-CENPB-N-22

Plasmid

#55957

Purpose

Localization: Nucleus/Centromeres, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Publication

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mPlum

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-CENPB-N-22

Plasmid

#54037

Purpose

Localization: Nucleus/Centromeres, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mEmerald

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pIBB-mEGFP-IRES-puro2b

Plasmid

#26462

Depositor

Daniel Gerlich

Publication

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pIBB

Tags

mEGFP

Expression

Bacterial and Mammalian

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLV_[Exp]-Hygro-EF1A-FUCCIplex

Plasmid

#240160

Purpose

Multiplexable FUCCI sensor (FUCCIplex). The EF1α promoter drives the expression of the fluorescent sensor.

Depositor

Francesco Pasqualini

Publication

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

EF1A

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCA12-pHR-SFFV-BFP-B23

Plasmid

#199443

Purpose

Expression of BFP-B23 in mammalian cells

Depositor

Baohui Chen

Publication

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

B23 (NPM1 Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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14x PP7 with loxP-kanMX-loxP

Plasmid

#189939

Purpose

Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

Depositor

Tineke Lenstra

Publication

Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

14x PP7 with loxP-kanMX-loxP

Tags

kanMX

Expression

Bacterial

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-FLAG-APEX2-LGALS8

Plasmid

#175758

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

Depositor

Wade Harper

Publication

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS8 (LGALS8 Human)

Use

Lentiviral

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-mRuby3-6xFLAG

Plasmid

#161613

Purpose

Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

Depositor

Edward Boyden

Publication

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

mRuby3-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mMaroon-KIX-mMaroon

Plasmid

#137009

Purpose

AAV backbone, mMaroon-KIX acceptor for R-CREB

Depositor

Ryohei Yasuda

Publication

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

AAV

Tags

mMaroon

Expression

Mammalian

Promoter

hSynapsin

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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BAS288 C terminal mKO2 natR

Plasmid

#74083

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

Depositor

Bassem Al-Sady

Publication

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223-MPP7

Plasmid

#23411

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MPP7 (MPP7 Human)

Use

Gateway Cloning

Mutation

2MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSEMS-Tom20-mEGFP-reHaloTagF

Plasmid

#200190

Purpose

Tom20 with reversible HaloTagF variant for fluorescent labeling

Depositor

Jacob Piehler

Publication

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Tags

mEGFP and reHaloTagF

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAG876 pDisplay-pFAST

Plasmid

#172868

Purpose

expresses pFAST at the surface of mammalian cells

Depositor

Arnaud Gautier

Publication

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST-PDGFR

Tags

IgK leader sequence (N terminal on insert) and My…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAMV:nAChRgamma-eGFP

Plasmid

#29693

Depositor

Henry Lester

Publication

Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Insert

GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)

Tags

AMV promotor and poly(A) tail

Expression

Bacterial

Mutation

eGFP inserted after position S380

Available since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1_BRCT W706RW815R

Plasmid

#250355

Purpose

Expresses mutant tandem BRCT domain from MCPH1 fused to eGFP. Serves as control.

Depositor

Tuncay Baubec

Publication

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Mutation

W706R and W815R

Promoter

CAGGS

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

Plasmid

#205022

Purpose

mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

Plasmid

#202608

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-WPRE

Plasmid

#202611

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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N-WASP

Plasmid

#186132

Purpose

For expression of recombinant N-WASp in bacteria cells.

Depositor

Michael Rosen

Publication

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

WASL (WASL Rat)

Tags

His6

Expression

Bacterial

Mutation

Amino Acid residues 183-193 fused to 273-501

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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