We narrowed to 5,561 results for: donor

1. pMC_cherry donor_Polb target (pE038)

   Plasmid

   #149349

   Purpose

   Replace donor vector for cherry insertion using the human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   cherry
   

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HoxC-downstream-5xTetO Donor

   Plasmid

   #131341

   Purpose

   Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   HoxC-downstream-5xTetO Donor
   

   Use

   CRISPR and Mouse Targeting

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mGDF8 donor CFP-hDF

   Plasmid

   #72590

   Purpose

   Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGDF8 donor CFP-hDF

   Plasmid

   #72591

   Purpose

   Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-TIGHT-EGFP-backward donor

   Plasmid

   #22077

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223252

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE-TIGHT-EGFP-donor fw copy

   Plasmid

   #22074

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Dual-Guide-Donor-ShuttleOnly

   Plasmid

   #171098

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Mutation

   XbaI site added upstream of U6 promotor. GtoC mu…

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-idCas9-KRAB-Hygro donor

   Plasmid

   #199621

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Insert

   AAVS1-idCas9-KRAB-Hygro
   

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid

    #162277

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

    Depositor

    Kazuhisa Nakayama

    Article

    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

    Insert

    HiFi Cas9
    

    Use

    CRISPR

    Tags

    BPNLS and FLAG tag

    Expression

    Mammalian

    Mutation

    SpCas9 (R691A)

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229783

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229785

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Promoter

    no promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid

    #80768

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

    Plasmid

    #229789

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF10 and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

    Plasmid

    #229788

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF10 and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDonor-tBFP-NLS-Neo (Universal)

    Plasmid

    #80767

    Purpose

    Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Puro-T2A-mCitrine-hSmad2-donor

    Plasmid

    #203663

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    Smad2
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. BSD-P2A-TagRFP-hSmad1-donor

    Plasmid

    #203662

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    smad1 (SMAD1 Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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