We narrowed to 9,286 results for: Pol;

1. sgiRFP2

   Plasmid

   #113973

   Purpose

   Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgiRFP3

   Plasmid

   #113974

   Purpose

   Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFP.R373

   Plasmid

   #138243

   Purpose

   Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GKA).SaP-dpol*.(CEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBz106_CsoS2_MR

   Plasmid

   #140843

   Purpose

   His-tagged CsoS2 Middle Region (MR), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 Middle Region (MR)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   only amino acids 257-603

   Promoter

   T7

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz75_CB_operon_CsoS2_N3-N4

   Plasmid

   #140857

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-109 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz77_CB_operon_CsoS2_N3

   Plasmid

   #140859

   Purpose

   Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-108 and 220-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLz78_CB_operon_CsoS2_delNTD

   Plasmid

   #140860

   Purpose

   Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLz76_CB_operon_CsoS2_N4

   Plasmid

   #140858

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-189 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBz109_CsoS2_NTD

   Plasmid

   #140842

   Purpose

   His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 N-terminal domain (NTD)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 262-869

   Promoter

   T7

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sg1

    Plasmid

    #113966

    Purpose

    Single short guide RNA targeting GTATAGCATACATTATACG

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg1
    

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBz110_CsoS2_CTD

    Plasmid

    #140844

    Purpose

    His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 C-terminal domain (CTD)
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-594

    Promoter

    T7

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM136

    Plasmid

    #134177

    Purpose

    Bacterial expression of his-CENP-C 700-943

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

    Insert

    his-CENP-C 700-943 (CENPC Human)
    

    Expression

    Bacterial

    Mutation

    aa 700-943

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR207-OEP7(1–50)

    Plasmid

    #100601

    Purpose

    Gateway entry vector for AtOEP7(1–50)

    Depositor

    Yutaka Kodama

    Article

    Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

    Insert

    OEP7(1–50) (OEP7 Mustard Weed)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pst39-NST-clone14

    Plasmid

    #133421

    Purpose

    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

    Depositor

    Gerhard Wagner

    Insert

    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    ORF for Nip1 encodes aa 4-156 (not complete ORF),…

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA-dabAB2

    Plasmid

    #133012

    Purpose

    pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFE-dabAB2 C351A

    Plasmid

    #133005

    Purpose

    pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. YCe1885 HC_Kan_Kozak-ATG-MLS_p6

    Plasmid

    #100676

    Purpose

    part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    Kozak-ATG-MLS
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. YCe2735 HC_Kan_Kozak-ATG-IgkL_p6

    Plasmid

    #100697

    Purpose

    part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    Kozak_ATG_IgkL
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CTL2 (M2225R)

    Plasmid

    #96952

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 (M222R) of PIEZO1
    

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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