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MED12_pLX307
Plasmid#98350PurposeLentiviral expression of MED12DepositorInsertMED12 (MED12 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR1392QPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1_pLX307
Plasmid#98326PurposeLentiviral expression of CSNK1A1DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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ERBB2 gRNA (BRDN0001148320)
Plasmid#76378Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB-Halo,IRES-eGFP
Plasmid#164519PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-iGluSnFR.A184S
Plasmid#106192PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterGFAPAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V
Plasmid#106178PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterhSynapsinAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148673)
Plasmid#76377Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pUDP002
Plasmid#103872PurposeBroad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)DepositorInsertshph
Spcas9 D147Y P411T
UseCRISPRTagsNLSExpressionYeastMutationD147Y P411TPromoterArxula adeninivorans TEF1 and Ashbya gossypii (Er…Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRecLV105
Plasmid#107238PurposeA lentiviral vector for expression in mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUNO mouse CD274 + 3'UTR WT full length
Plasmid#107012PurposeExpresses murine PD-L1 with the 3'UTRDepositorInsertCD274 (Cd274 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Luciferase
Plasmid#213979PurposeExpresses Doxy Inducible LuciferaseDepositorInsertLuciferase
UseLentiviralTags6xHis affinity tagExpressionBacterial and MammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1B-WT-Flag-UTR
Plasmid#183239PurposeExpression of FLAG tagged HNF1BDepositorInsertHNF1 homeobox B (HNF1B Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
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RPS18_pLX307
Plasmid#98370PurposeLentiviral expression of RPS18DepositorInsertRPS18 (RPS18 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CCNT1_pLX307
Plasmid#98328PurposeLentiviral expression of CCNT1DepositorInsertCCNT1 (CCNT1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationH519R, S566P, and P712SPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABL1 gRNA (BRDN0001147582)
Plasmid#77732Purpose3rd generation lentiviral gRNA plasmid targeting human ABL1DepositorInsertABL1 (ABL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRECLV103_PGBD5_B
Plasmid#168053PurposeLentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti TMEM30A
Plasmid#171789Purposetransfer plasmid for lentiviral vector production expressing TMEM30ADepositorInsertTMEM30A (TMEM30A Human)
UseLentiviralTags6X His + FlagExpressionMutationPromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5 + PGK-puro
Plasmid#167817PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)
Plasmid#185915PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK4
Plasmid#98617PurposeGateway entry TOPO TA cloning vector for ELK4DepositorInsertELK4 (Elk4 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF251
Plasmid#101451PurposeDonor Vector containing ZNF251 transcription factor, part of the Human TFome CollectionDepositorInsertZNF251 (ZNF251 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS
Plasmid#127656PurposeOver-express NLS-FLAG-cGASDepositorInsertcGAS (CGAS Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mTagBFP2 rat PGK1 shRNA
Plasmid#222869PurposeExpresses mTagBFP2 along with an shRNA against rat PGK1DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
FERMT1_pLX307
Plasmid#98333PurposeLentiviral expression of FERMT1DepositorInsertFERMT1 (FERMT1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHOT-P2Y2R
Plasmid#159108Purposeentiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the GibsonDepositorInsertP2Y2R
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLgw Cbx1-V5-EcoDam
Plasmid#59211PurposeMammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.DepositorInsertCbx1 (Cbx1 Mouse)
UseLentiviral; DamidTagsDam and V5ExpressionMammalianMutationPromoterHeat Shock Minimal Promoter and heat shock minima…Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.A184V
Plasmid#106181PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterhSynapsinAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001149093)
Plasmid#77788Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GCH1_pLX307
Plasmid#98336PurposeLentiviral expression of GCH1DepositorInsertGCH1 (GCH1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mCherry-Rab11
Plasmid#140573PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorUseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only
