We narrowed to 25,502 results for: puro

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pcDNA3puro-EGFP-GAPDH

Plasmid

#225475

Purpose

Express mEGFP-fusion protein of GAPDH

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 C311S

Plasmid

#40985

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C311S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

Plasmid

#197989

Purpose

For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNT0217290

Use

Lentiviral

Tags

lac

Expression

Mammalian

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

Plasmid

#170467

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

Plasmid

#170322

Purpose

Expresses FLAG and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

Plasmid

#139447

Purpose

Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101108

Purpose

Encodes dCas9(N) fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-MRLC1-mRuby2-IRES-PuroR

Plasmid

#103031

Purpose

Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Expression

Mammalian

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

Plasmid

#129722

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-3XFlag

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_NS3_Flag

Plasmid

#79635

Purpose

Third generation Self-inactivating lentivector expressing NS3 from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS3

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_NS3_Flag

Plasmid

#79635

Purpose

Third generation Self-inactivating lentivector expressing NS3 from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS3

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pICE

Plasmid

#46960

Purpose

Plasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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