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Plasmid#119688PurposeFor mammalian expression of LIR mutant p62 without its UBDDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationW340A & UBD deletion (1-385)PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K243R
Plasmid#129301PurposeExpresses pCMV 3xFLAG-ATG3 K243R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 243 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL2
Plasmid#89295PurposeFor expression of the mammalian Atg8 protein GABARAPL2 without a tagDepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-muGFP-hATG2A mLIR
Plasmid#215506PurposeExpresses GFP tagged human ATG2A with the mutation in LC3 interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…PromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 C264A
Plasmid#129293PurposeGateway entry clone encoding human ATG3 C264A (catalytic inactive)DepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged Cysteine 264 to AlaninePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 K243R
Plasmid#129294PurposeGateway entry clone encoding human ATG3 K243RDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged Lysine 243 to ArgininePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 allKR
Plasmid#129295PurposeGateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginineDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged all 22 Lysine residues to ArgininePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 allKR
Plasmid#129298PurposeExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged all 22 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K295R
Plasmid#129302PurposeExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 295 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hATG7CS
Plasmid#87868PurposeExpress a active-site mutant human Atg7/Apg7 C572S in mammalian cellsDepositorInsertATG7 C572S mutant (ATG7 Human)
UseTagsExpressionMammalianMutationC572S mutantPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-muGFP-hATG2A mLIRΔWIR
Plasmid#215508PurposeExpresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…PromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 1-13 KR
Plasmid#129299PurposeExpresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged first 13 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 14-22 KR
Plasmid#129300PurposeExpresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged last 9 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMU-AUTr
Plasmid#61204PurposeFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoterDepositorInsertLC3(ATG8)
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-AUTr
Plasmid#61205PurposeFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoterDepositorInsertLC3(ATG8)
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)
Plasmid#169076PurposeInsect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepIIDepositorInsertsUseTagsGGlinker, TEV cleavage site, StrepII and HIS10 Ta…ExpressionBacterial and InsectMutationPromoterPolyhedrin promoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B-LIRmut
Plasmid#109027Purposestable lentiviral expression of cDNADepositorInsertFAM134B-LIRmut (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDDFELL to DDAEAL aa 453-458 to interrupt FAM134B …PromoterEIF-1a promoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)
Plasmid#169077PurposeInsect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepIIDepositorInsertsUseTagsGFP tag, GGlinker, TEV cleavage site. StrepII and…ExpressionBacterial and InsectMutationPromoterPolyhedrin promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-GFP-NEK9W967A
Plasmid#168271PurposeExpresses LIR (LC3-interacting region) mutant NEK9DepositorInsertNEK9 (NEK9 Human)
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 979 to alaninePromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::lgg-1 (lgg-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only