N-linked glycosylation regulates SNAT2 trafficking and stability in pancreatic ductal adenocarcinoma cells.
Koe JC, Pashaoskooie K, Johal D, Zhong YC, Wu C, Parker SJ
Am J Physiol Cell Physiol. 2026 Jun 29. doi: 10.1152/ajpcell.00060.2026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237226 | pLenti CMV SNAT2-eGFP WT (wildtype) | Lentiviral destination vector expressing sgRNA-resistant wildtype SNAT2 (SLC38A2) cDNA containing C-terminal eGFP. |
| 237227 | pLenti CMV SNAT2-eGFP 3NQ | Lentiviral destination vector expressing sgRNA-resistant SNAT2 (SLC38A2) cDNA containing C-terminal eGFP. SNAT2 sequence contains mutations to inhibit N-linked glycosylation. |
| 237228 | pInducer20 SNAT2-eGFP WT (wildtype) | Lentiviral vector expressing dox-inducible sgRNA-resistant wildtype SNAT2 (SLC38A2) cDNA containing C-terminal eGFP. |
| 237229 | pInducer20 SNAT2-eGFP 3NQ | Lentiviral vector expressing dox-inducible sgRNA-resistant SNAT2 (SLC38A2) cDNA containing C-terminal eGFP. SNAT2 sequence contains mutations to inhibit N-linked glycosylation. |
| 237230 | pLenti CRISPRv2 sgSLC38A2#1 | Plasmid for SNAT2 (SLC38A2) CRISPR knockout in human cell lines. |
| 237231 | pDONR221 SNAT2-eGFP WT (wildtype) | Donor vector for gateway cloning of sgRNA-resistant wildtype SNAT2 (SLC38A2) cDNA sequence containing C-terminal eGFP. |
| 237232 | pDONR221 SNAT2-eGFP 3NQ | Donor vector for gateway cloning of sgRNA-resistant SNAT2 (SLC38A2) cDNA sequence containing C-terminal eGFP. SNAT2 sequence contains mutations to inhibit N-linked glycosylation. |