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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,525 results for: golden gate

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Showing: 5001 - 5020 of 5525 results

  1. pCHW91069

    Plasmid
    #222462
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Publication
    Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-miniDFR (Solanum lycopersicum)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222462-plasmid-map-sequence-id-454068

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  4. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Publication
    Najia et al Cell. 2026 Sep 1:S0092-8674(26)00932-3.
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

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  6. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Richard Murray
    Publication
    Kapasiawala et al ACS Synth Biol. 2024 Dec 20;13(12):3976-
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227645-plasmid-map-sequence-id-452394

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  19. pJB67

    Plasmid
    #226316
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Publication
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with K to A CsoS2 MR1-6
    Expression
    Bacterial
    Mutation
    K261A, K320A, K380A, K436A, K495A, K546A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226316-plasmid-map-sequence-id-449928

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  20. pJB68

    Plasmid
    #226315
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Publication
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1-7
    Expression
    Bacterial
    Mutation
    Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226315-plasmid-map-sequence-id-449927

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Showing: 5001 - 5020 of 5525 results