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PB-NLS-ZapCV2

Plasmid

#203664

Purpose

Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

Depositor

Amy Palmer

Publication

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2 Zinc sensor

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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PB-NLS-ZapCV2-ALA

Plasmid

#203665

Purpose

Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

Depositor

Amy Palmer

Publication

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Publication

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCBP60g-NLS-3xmCitrine

Plasmid

#192521

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Publication

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

CALMODULIN BINDING PROTEIN 60g

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

CBP60g

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-Stim1-D76A

Plasmid

#186622

Purpose

Encodes Stim1 mutant D76A fused to YFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

D76A

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YFP-Stim1-ΔCTD

Plasmid

#186621

Purpose

Encodes Stim1 with C-terminal domain deletion fused to YFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Deletion of C-terminal domain 601 to 685

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSelect-6

Plasmid

#190243

Purpose

Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Publication

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEA183

Plasmid

#187873

Purpose

Expresses eGFP under the virB promoter.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

eGFP

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFTR5

Plasmid

#167507

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PTetA promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFVK5

Plasmid

#167522

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBVR5

Plasmid

#167511

Purpose

Broad-Host range expression vector, pBBR origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFTtR5

Plasmid

#167508

Purpose

Broad-Host range expression vector, RSF1010 origin, TtgRAM repressor, PTtg promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTtg

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFRG5

Plasmid

#149486

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1050E (Ubiq-CasRx)

Plasmid

#132416

Purpose

Express CasRx under Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

CasRx

Expression

Insect

Promoter

Ubiq

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDCB014

Plasmid

#135433

Purpose

Yeast integrating plasmid to express MCP-ABI and PYL1-VP64

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Inserts

MCP-ABI
PYL1-VP64

Expression

Yeast

Promoter

pAdh1 and pHHF2

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC533

Plasmid

#135435

Purpose

Yeast integrating plasmid for pTEF1-mCherry reporter gene

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

mCherry

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

pTEF1

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Publication

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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