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EF1a_KLF9_P2A_Hygro_Barcode

Plasmid

#120495

Purpose

Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF9 (KLF9 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF2_P2A_Hygro_Barcode

Plasmid

#120489

Purpose

Barcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF2 (KLF2 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xControl

Plasmid

#245729

Purpose

Express 42xControl mRNA in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xControl mRNA

Expression

Mammalian

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xRCGG/CGA/AGG

Plasmid

#245728

Purpose

Express 42xRCGG/CGA/AGG mRNA in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xRCGG/CGA/AGG

Expression

Mammalian

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-E95A-Tev-3xFlag

Plasmid

#245726

Purpose

Express CNOT3-E95A-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-E95A-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

E95A

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-Tev-3xFlag

Plasmid

#245724

Purpose

Express CNOT3-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-K105S-Tev-3xFlag

Plasmid

#245727

Purpose

Express CNOT3-K105S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-K105S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

K105S

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-R59S-Tev-3xFlag

Plasmid

#245725

Purpose

Express CNOT3-R59S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-R59S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

R59S

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBetaActin-IC2N-dCer3

Plasmid

#191325

Purpose

Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

IC2N-dCer-FRB (Dync1i2 Mouse)

Expression

Mammalian

Mutation

aa 1-237

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Expression

Mammalian

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKC530 pBABE EGFP-ZNF335

Plasmid

#192698

Purpose

Retroviral transgene expression of human ZNF335

Depositor

Iain Cheeseman

Publication

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

ZNF335 (ZNF335 Human)

Use

Retroviral

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S10

Plasmid

#128594

Purpose

Expresses tagless S10 ribosomal protein in E.coli

Depositor

James Williamson

Publication

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S10 (rpsJ E.coli)

Expression

Bacterial

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3

Plasmid

#128591

Purpose

Expresses tagless S3 ribosomal protein in E.coli

Depositor

James Williamson

Publication

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S3 E.coli ribosomal protein (rpsC E.coli)

Expression

Bacterial

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylI

Plasmid

#48200

Purpose

Expresses CylI (type III PKS) in E. coli

Depositor

Emily Balskus

Publication

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylI

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET-29b-CylB

Plasmid

#48199

Purpose

Expresses CylB (acyl carrier protein) in E. coli

Depositor

Emily Balskus

Publication

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylB

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5-ratERK2-I84A

Plasmid

#40815

Depositor

Natalie Ahn

Publication

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

I84A

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB-gDA3mut

Plasmid

#208704

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_gDA3mut in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) control sensor GRAB_gDA3mut

Use

AAV

Promoter

hSyn

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB-rDA3m

Plasmid

#208708

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent manner

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) IGF1R

Plasmid

#107499

Purpose

Expresses IGF1R, puromycin resistant

Depositor

Alejandro Gutierrez

Publication

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

IGF-1R (IGF1R Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pNX3c2-FL3

Plasmid

#78391

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
natCX

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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