We narrowed to 15,622 results for: pET

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bla-A OR T-SpyTag

Plasmid

#246945

Purpose

Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199

Insert

Please see depositor comments below

Tags

6x His tag

Expression

Bacterial

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-S-SpyTag

Plasmid

#243786

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCherry

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A-SpyTag

Plasmid

#243769

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S-SpyTag

Plasmid

#243770

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T-SpyTag

Plasmid

#243771

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR S-SpyTag

Plasmid

#243772

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR T-SpyTag

Plasmid

#243773

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S OR T-SpyTag

Plasmid

#243774

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A AND T-SpyTag

Plasmid

#243776

Purpose

Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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MZB139

Plasmid

#217838

Purpose

Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

SMT3 (SMT3 Budding Yeast)

Tags

10x His and SUMO (SMT3)

Expression

Bacterial

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB164

Plasmid

#217842

Purpose

10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

HaloTag

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Promoter

T7 Promoter

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSRK51

Plasmid

#223722

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

Sreejith Raran-Kurussi

Article

Parida et al Curr Res Struct Biol. 2023 Sep 30;6:1001

Insert

TEV protease

Tags

NT* (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

T7

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP580

Plasmid

#222959

Purpose

Expresses R141S K142S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine and Lysine 142 to …

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP579

Plasmid

#222958

Purpose

Expresses R36S K51S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine and Lysine 51 to Se…

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT203

Plasmid

#204512

Purpose

Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

C04F12.1 (C. elegans)

Tags

6xHis, MBP

Expression

Bacterial

Promoter

T7lac

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT517

Plasmid

#204518

Purpose

Bacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

cec-4 (C. elegans)

Tags

6xHis, MBP

Expression

Bacterial

Promoter

T7lac

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLz54_polyPro_polar

Plasmid

#140847

Purpose

Same as pLz47 but with 20 polar amino acids instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with polar residues

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz58_polyPro_CsoS2_CTP

Plasmid

#140850

Purpose

Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 C-terminal peptide

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz33_CsoS2_NTD_GFP

Plasmid

#140861

Purpose

His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD fused to GFP

Tags

Hexahistidine tag and Superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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