We narrowed to 14,926 results for: pET

1. pACB110

   Plasmid

   #222209

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (G258D)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Gly258 to Aspartate

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GB1-ΔN-CHMP4C(G154C)

   Plasmid

   #190783

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   G154C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-ΔN-CHMP4C(M165C)

   Plasmid

   #190784

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   M165C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-ΔN-CHMP4C(S191C)

   Plasmid

   #180027

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   S191C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBtPIPLC_N168C His

   Plasmid

   #173809

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Asn168Cys for labeling

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3R112C/T180CHFm

   Plasmid

   #85091

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C in PCNA3, A74G in heme-FMN domain of …

   Promoter

   T7 promoter

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HASP_HUMAN_D0

   Plasmid

   #79730

   Purpose

   This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   HASP
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. KCC2D_HUMAN_D0

   Plasmid

   #79734

   Purpose

   This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC2D
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. AAPK2_HUMAN_D0

   Plasmid

   #79738

   Purpose

   This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   AAPK2
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. KCC4_HUMAN_D0

    Plasmid

    #79744

    Purpose

    This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC4 (SLC12A7 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. KCC1G_HUMAN_D0

    Plasmid

    #79745

    Purpose

    This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC1G
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. KCC2A_HUMAN_D0

    Plasmid

    #79746

    Purpose

    This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2A
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. KC1G2_HUMAN_D0

    Plasmid

    #79716

    Purpose

    This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G2
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. STK10_HUMAN_D0

    Plasmid

    #79722

    Purpose

    This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK10 (STK10 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. MK03_HUMAN_D0

    Plasmid

    #79688

    Purpose

    This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK03
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. PAK4_HUMAN_D0

    Plasmid

    #79698

    Purpose

    This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PAK4 (PAK4 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. PAK6_HUMAN_D0

    Plasmid

    #79703

    Purpose

    This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PAK6 (PAK6 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. HL 716

    Bacterial Strain

    #60368

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. HL 770

    Bacterial Strain

    #60369

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HL 1128

    Bacterial Strain

    #60372

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only