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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 5101 - 5120 of 6962 results

  1. pcDNA3_N-DYK_MTHFD1-NLS

    Plasmid
    #133023
    Purpose
    NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)
    Depositor
    Stefan Kubicek
    Publication
    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MTHFD1 (MTHFD1 Human)
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    133023-plasmid-map-sequence-id-338753

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  5. PRMT8(E185Q)-FLAG

    Plasmid
    #164700
    Purpose
    Expression of PRMT8(E185Q) with a C-terminal FLAG tag
    Depositor
    Cheryl Arrowsmith
    Insert
    PRMT8 (PRMT8 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E185Q
    Promoter
    CMV
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164700-plasmid-map-sequence-id-326681

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  8. BE3-NLS-NLS-P2A-BLAST

    Plasmid
    #135348
    Purpose
    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135348-plasmid-map-sequence-id-267697

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  10. SpyCas9-AIDx

    Plasmid
    #135343
    Purpose
    Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpyCas9-hAIDx
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135343-plasmid-map-sequence-id-267693

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  11. pcDNA3-HCMVgH (Merlin)

    Plasmid
    #136448
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136448-plasmid-map-sequence-id-275046

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  13. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid
    #136452
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136452-plasmid-map-sequence-id-269876

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  14. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid
    #136453
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136453-plasmid-map-sequence-id-271682

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  15. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid
    #136447
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-716). Codon-optimize…
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136447-plasmid-map-sequence-id-273651

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  16. pcDNA3-sACE2v2.2-sfGFP

    Plasmid
    #149665
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    149665-plasmid-map-sequence-id-298416

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  17. pcDNA3-sACE2v2.2-IgG1

    Plasmid
    #149667
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    149667-plasmid-map-sequence-id-298414

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  19. pcDNA3-sACE2v6-Fc(IgA1)

    Plasmid
    #145161
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgA1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145161-plasmid-map-sequence-id-292608

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  20. pcDNA3-sACE2v5-Fc(IgG1)

    Plasmid
    #145168
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 5
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145168-plasmid-map-sequence-id-292627

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Showing: 5101 - 5120 of 6962 results