Aurora kinase A localises to mitochondria to control organelle dynamics and energy production.
Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier M
Elife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111. PubMed Article
Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier M
Elife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157749 | pcDNA 3.1 AURKA deltaNter | Expression of AURKA without Nter in mammalian cells |
| 157750 | pcDNA 3.1 mitoAURKA | Expression of AURKA targeted to mitochondria |
| 157751 | pcDNA 3.1 AURKA Lys162Met | Expression of kinase-dead AURKA K62M in mammalian cells |
| 157752 | pcDNA 3.1 mitoAURKA Lys162Met | Expression of kinase-dead AURKA K62M in mitochondria |
| 157753 | pEGFP N1 AURKA deltaNter | Expression of AURKA without Nter, fused to GFP in mammalian cells |
| 157754 | pEGFP N1 mitoAURKA | Expression of AURKA:GFP targeted to mitochondria |
| 157755 | pEGFP N1 AURKA 1-110 | Expression of AURKA Nter (aa1-110):GFP in mammalian cells |
| 157756 | pEGFP N1 AURKA 1-30 | Expression of AURKA Nter (aa1-30):GFP in mammalian cells |
| 157757 | pCMV7 mitoDendra2 | Expression of the fluorescent protein Dendra2 targeted to mitochondria |
| 157758 | pmCherry C1 Omp25 | Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells |
| 157759 | pmCherry N1 MFN2 | Expression of MFN2:mcherry in cells |
| 157760 | pmCherry C1 MFF | Expression of mcherry:MFF in cells |
| 157761 | pmCherry N1 TOMM20 | Expression of TOMM20:mcherry in cells |
| 157762 | pmCherry N1 TOMM22 | Expression of TOMM22:mcherry in cells |
| 157767 | pCMV AURKA-mTurq2 | Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter |
| 157768 | pCMV ShadowG-AURKA-mTurq2 | Expression of AuroraA kinase biosensor under CMV promoter |
| 157769 | pCMV ShadowG-AURKA K162M-mTurq2 | Expression of AuroraA kinase-dead biosensor under CMV promoter |
| 157770 | pCMV ShadowY-AURKA-mTurq2 | Expression of AuroraA kinase biosensor under CMV promoter |
| 157771 | pCMV ShadowY-AURKA K162M-mTurq2 | Expression of AuroraA kinase-dead biosensor under CMV promoter |
| 157772 | pCMV superYFP-AURKA-mTurq2 | Expression of AuroraA kinase biosensor under CMV promoter |
| 157773 | pCMV superYFP-AURKA K162M-mTurq2 | Expression of AuroraA kinase-dead biosensor under CMV promoter |
| 157774 | pCMV mTurquoise2-shadowG tandem | Produces a fusion between mTurquoise2 and shadowG. It can be used as a positive control for FRET (using FLIM) |
| 157775 | pCMV mTurquoise2-shadowY tandem | Produces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM) |
| 157776 | pCMV mTurquoise2-superYFP tandem | Produces a fusion between mTurquoise2 and superYFP. It can be used as a positive control for FRET (using FLIM) |