Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions.
Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131171pOVC1Cloning vector containing A-B-C casette
Loading...
131172pOVC2Cloning vector containing A-C-B casette
Loading...
131173pOVC3Cloning vector containing B-A-C casette
Loading...
131174pOVC1_VfAU1-LOV_XExpresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites
Loading...
131175pOVC1_CrPH-LOV_XExpresses CrPh-LOV domain (Chlamydomonas reinhardtii) followed by restriction sites
Loading...
131176pOVC1_NcVVD-LOV_XExpresses NcVVD-LOV (Neurosporra crassa) followed by restriction sites
Loading...
131177pOVC1_RsLP-LOV_XExpresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites
Loading...
131178pOVC1_MxCarH-CBD_XExpresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites
Loading...
131179pOVC1_TtCarH-CBD_XExpresses TtCarH-CBD (Thermus termophilus) followed by restriction sites
Loading...
131180pOVC1_AtCRY2-PHR_XExpresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sites
Loading...
131181pOVC1_AtCIB_XExpresses AtCIB (Arabidopsis thaliana) followed by restriction sites
Loading...
131182pOVC1_AtPIF6_XExpresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites
Loading...
131183pOVC1_AtPHYB-S_XExpresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites
Loading...
131184pOVC1_ScPH1-S_XExpresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites
Loading...
131185pOVC1_X_VfAU1-LOVExpresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites
Loading...
131186pOVC1_X_CrPH-LOVExpresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites
Loading...
131187pOVC1_X_NcVVD-LOVExpresses NcVVD-LOV (Neurosporra crassa) preceded by restriction sites
Loading...
131188pOVC1_X_RsLP-LOVExpresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sites
Loading...
131189pOVC1_X_MxCarH-CBDExpresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sites
Loading...
131190pOVC1_X_TtCarH-CBDExpresses TtCarH-CBD (Thermus termophilus) preceded by restriction sites
Loading...
131191pOVC1_X_AtCRY2-PHRExpresses AtCRY2-PHR (Arabidopsis thaliana) preceded by restriction sites
Loading...
131192pOVC1_X_AtPIF6Expresses AtPIF6 (Arabidopsis thaliana) preceded by restriction sites
Loading...
131193pOVC1_X_ScPH1-SExpresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites
Loading...
131194pOVC1_X_AtCIBExpresses AtCIB (Arabidopsis thaliana) preceded by restriction sites
Loading...
131208pOVC1_L1(GSSSG)Cloning vector containing A-B-C casette with linker L1 in site B
Loading...
131209pOVC1_L2(GSSGGSSG)Cloning vector containing A-B-C casette with linker L2 in site B
Loading...
131210pOVC1_L3(GSSSSGGSSSG)Cloning vector containing A-B-C casette with linker L3 in site B
Loading...
131211pOVC1_L4(EAAAK)3Cloning vector containing A-B-C casette with linker L4 in site B
Loading...
131212pOVC1_EcSSPB-nano_XExpresses EcSSPB-nano (Escherichia coli) followed by restriction sites
Loading...
131213pOVC1_EcSSPB-micro_XExpresses EcSSPB-micro (Escherichia coli) followed by restriction sites
Loading...
131214pOVC1_HsPDZ1b_XExpresses HsPDZ1b (Homo sapiens) folllwed by restriction sites
Loading...
131215pOVC1_X_AsPT1-LOV2-pepExpresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites
Loading...
131216pOVC1_X_HsPDZ1bExpresses HsPDZ1b (Homo sapiens) folllwed by restriction sites
Loading...
131217pOVC1_X_AsPT1-LOV2-EcSsraExpresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites
Loading...
131218pOVC1_X_EcSSPB-microExpresses EcSSPB-nano (Escherichia coli) preceded by restriction sites
Loading...
131219pOVC1_X_EcSSPB-nanoExpresses EcSSPB-micro (Escherichia coli) preceded by restriction sites
Loading...
131220Opto-casp9_VfAU1-LOV_casp9Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)
Loading...
131221Opto-casp9_AtCRY2-PHR-casp9Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)
Loading...
131222Opto-casp9_casp9-VfAU1-LOVExpresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9)
Loading...
131223Opto-casp9_casp9-AtCRY2-PHROpto-casp9_casp9-AtCRY2-PHR (light-activated caspase9)
Loading...
131224VfAU1-LOV_P2A_casp9Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site
Loading...

Antibodies from Article