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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 5141 - 5160 of 14352 results

  1. pEvolvR-mCherry-enCas9(D10A)-PolI3M

    Plasmid
    #249061
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enCas9-PolI3M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249061-plasmid-map-sequence-id-495618

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  2. pEvolvR-mCherry-enCas9(H840A)-PolI5M

    Plasmid
    #249064
    Purpose
    Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enCas9(H840A)-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249064-plasmid-map-sequence-id-495619

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  3. pYJ28-AAVS1-XLone-dCas9-p300core

    Plasmid
    #131071
    Purpose
    Donor plasmid of targeted inducible-dCAS9-P300core knockin
    Depositor
    Xiaojun Lian
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-p300core
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131071-plasmid-map-sequence-id-270718

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  11. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid
    #183924
    Purpose
    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer
    Depositor
    Karsten Rippe
    Publication
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GFP4-VPR
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183924-plasmid-map-sequence-id-364146

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  17. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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Showing: 5141 - 5160 of 14352 results