Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,968 results for: Kars

Showing: 5141 - 5160 of 15968 results

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pAAV hPGRN

Plasmid

#213682

Purpose

Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Human Progranulin (hPGRN) (GRN Human)

Use

AAV

Tags

Twin-Strep tag and V5 tag (after signal peptide)

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-NpHR3.3-EYFP

Plasmid

#137152

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137141

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-NpHR3.3-EYFP

Plasmid

#137154

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

Plasmid

#137120

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP

Plasmid

#137148

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

Plasmid

#137163

Purpose

Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (GAPDH)

Plasmid

#180183

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137139

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP

Plasmid

#137153

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP

Plasmid

#137149

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-Arch3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

Plasmid

#137146

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Con/Foff 2.0-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

Plasmid

#137150

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

Plasmid

#137143

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

Plasmid

#159577

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

Plasmid

#159578

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKlab1157-HBEGF-2a-3xFLAG-BRCA1(full-length)-P1749S-2a-mRuby3-HBBIVS2-WPRE

Plasmid

#248997

Purpose

This plasmid expresses the BRCA1 sequence with P1749S mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Use

CRISPR

Tags

3x-FLAG-V5

Mutation

P1749S

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab1158-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699Q-2a-mRuby3-HBBIVS2-WPRE

Plasmid

#248998

Purpose

This plasmid expresses the BRCA1 sequence with R1699Q mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Use

CRISPR

Tags

3x-FLAG-V5

Mutation

R1699Q

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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Showing: 5141 - 5160 of 15968 results

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