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pLMB684

Plasmid

#83779

Purpose

Xylose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

upstream region of RL2720 (rbsC)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Otx2shRNA1

Plasmid

#73981

Purpose

Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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CRISPRmTmG2

Plasmid

#69992

Purpose

The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA that targets near LoxP sites

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSR-puro-Sec5

Plasmid

#31117

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Sec5 shRNA (EXOC2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
pAV0773

Plasmid

#133519

Purpose

Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLMB687

Plasmid

#83781

Purpose

Sucrose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL120556 containing all of pRL120555

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PFD1130w-bio-His

Plasmid

#50823

Purpose

Expresses enzymatically monobiotinylated full length PFD1130w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFD1130w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE658

Plasmid

#153256

Purpose

M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9B-V5

Plasmid

#35938

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Tags

V5

Expression

Mammalian

Mutation

SNP Met106-->Thr

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

Plasmid

#171689

Purpose

Flp-dependent expression of jGCaMP7b

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

GCaMP7b

Use

AAV

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1C389S-FKBP

Plasmid

#108130

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S:FKBP(3-108) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT1

Plasmid

#60038

Purpose

Mammalian expression of FLAG-tagged mouse TUT1

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT1 (Mtpap Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-100u

Plasmid

#13547

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F463A

Plasmid

#45975

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F463A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pIS47 C20orf139 3'UTR

Plasmid

#14489

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
SERA7-bio-His

Plasmid

#50819

Purpose

Expresses enzymatically monobiotinylated full length SERA7

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA7

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2 DN-xIGFR

Plasmid

#24699

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Dominant negative xIGFR (igf1r Frog)

Expression

Mammalian

Mutation

C-terminal truncated version of the Xenopus IGF t…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pJOG331

Plasmid

#105408

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHA tag fused to Twin-Strep tag

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Wnt2B2 Non-STOP

Plasmid

#35889

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2B2 (WNT2B Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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ROZA

Plasmid

#64198

Purpose

"Reporter of Zap-70 Activity" Fluorescent reporter of ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only