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AB.pCCL.sin.cPPT.U6.miR-128-Decoy.hPGK.GFP.WPRE

Plasmid

#46584

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-128 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pML2(+)BR

Plasmid

#108336

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM390

Plasmid

#84202

Purpose

Disturb the expression of miR390 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR390

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM393

Plasmid

#84203

Purpose

Disturb the expression of miR393 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR393

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM156

Plasmid

#84194

Purpose

Disturb the expression of miR156 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR156

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-16-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85876

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16-2-3p Sensor (MIR16-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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VCDstub-GFP

Plasmid

#33122

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
p201N 1514

Plasmid

#47025

Purpose

Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Inserts

gma-miR1514 recognition sequence
nptII selectable marker

Use

RNAi

Promoter

GmUbi and StUbi-3

Available Since

Aug. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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AB.pCCL.sin.cPPT.U6.miR-718-Decoy.hPGK.GFP.WPRE

Plasmid

#46632

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-718 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-211-Decoy.hPGK.GFP.WPRE

Plasmid

#46600

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-211 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
ΔCT190-GFP

Plasmid

#33118

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 190 aa of the variable cytop…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-675-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46631

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-675-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-1306-Decoy.hPGK.GFP.WPRE

Plasmid

#46585

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-1306 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
EF1a-Triplex-28-M13-28-pA

Plasmid

#202041

Purpose

Encodes a non-coding transcript expressed from the EF1a promoter that has a 3' UTR containing a MALAT1 triplex, Csy4 binding sites ("28"), and a cloning site for a Cas9 gRNA.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

Triplex-28-M13-28 (noncoding)

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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