Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,873 results for: gibson assembly

Clear keywords
Showing: 501 - 520 of 1873 results

  2. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

    Enlarge

  3. pV2A-T

    Plasmid
    #153022
    Purpose
    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter
    Depositor
    Vito Valiante
    Article
    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    153022-plasmid-map-sequence-id-314021

    Enlarge

  4. pV2A-T

    Plasmid
    #153022
    Purpose
    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter
    Depositor
    Vito Valiante
    Article
    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    153022-plasmid-map-sequence-id-314021

    Enlarge

  11. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

    Enlarge

  12. YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A)

    Plasmid
    #228886
    Purpose
    AAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-
    Insert
    GEMINI(A); GEMINI(B); HaloTag-GEMINI(A)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    hSyn; Ubc
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228886-plasmid-map-sequence-id-454809

    Enlarge

  17. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

    Enlarge

Showing: 501 - 520 of 1873 results