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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,029 results for: gibson assembly

Showing: 501 - 520 of 1029 results

  1. pFP.R374

    Plasmid
    #138244
    Purpose
    Expresses the split mCherry reporter interrupted by the SaP-Hel artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGK).SaP-Hel*.(TSS)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138244-plasmid-map-sequence-id-273478

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  2. pFP.R355

    Plasmid
    #138225
    Purpose
    Expresses the split mCherry reporter interrupted by the Cro-RIR1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(VNQ).Cro-RIR1*.(SGR)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138225-plasmid-map-sequence-id-273520

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  3. pFP.R356

    Plasmid
    #138226
    Purpose
    Expresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(DKF).Cro-RPB2*.(STK)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138226-plasmid-map-sequence-id-273504

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  4. pFP.R357

    Plasmid
    #138227
    Purpose
    Expresses the split mCherry reporter interrupted by the Cro-Top2 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GSK).Cro-Top2*.(CSN)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138227-plasmid-map-sequence-id-273517

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  5. pFP.R358

    Plasmid
    #138228
    Purpose
    Expresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).CIV-RIR1*.(CSE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138228-plasmid-map-sequence-id-273508

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  6. pFP.R359

    Plasmid
    #138229
    Purpose
    Expresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(KHH).CP-Thy1*.(SVL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138229-plasmid-map-sequence-id-273505

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  7. pFP.R361

    Plasmid
    #138231
    Purpose
    Expresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).BsuP-RIR1*.(CSE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138231-plasmid-map-sequence-id-273489

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  8. AP553‐1

    Plasmid
    #87240
    Purpose
    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87240-plasmid-map-sequence-id-173227

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  10. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI site removed
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  11. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

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  12. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  13. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  14. C71m

    Plasmid
    #120983
    Purpose
    MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    CinR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120983-plasmid-map-sequence-id-236042

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  15. pBP-TagR_MCD22

    Plasmid
    #204104
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD22 weak RBS for assembly with tag part
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204104-plasmid-map-sequence-id-413905

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  16. pBP-TagR_MCD8

    Plasmid
    #204105
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD8 weak RBS for assembly with tag part
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204105-plasmid-map-sequence-id-413906

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  17. pBP-TagR_MCD10

    Plasmid
    #204101
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD10 medium RBS for assembly with tag part
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204101-plasmid-map-sequence-id-413902

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  18. pBP-TagR_MCD7

    Plasmid
    #204102
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD7 medium RBS for assembly with tag part
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204102-plasmid-map-sequence-id-413903

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  19. pBP-TagR_MCD15

    Plasmid
    #204103
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD15 medium RBS for assembly with tag part
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204103-plasmid-map-sequence-id-413904

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  20. pBP-TagR_MCD2

    Plasmid
    #204098
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD2 strong RBS for assembly with tag part
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204098-plasmid-map-sequence-id-413899

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Showing: 501 - 520 of 1029 results