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pGLU_385

Plasmid

#250620

Purpose

Himar.004 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Publication

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.004

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_386

Plasmid

#250621

Purpose

Himar.005 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Publication

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.005

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_387

Plasmid

#250622

Purpose

Himar.007 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Publication

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.007

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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lvl2-[lenti-backbone]

Plasmid

#229208

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Publication

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-E1

Plasmid

#229161

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Publication

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

E1

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-F1

Plasmid

#229162

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Publication

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

F1

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJMH036

Plasmid

#186637

Purpose

aadA.co between homology arms for NSI

Depositor

Adam Arkin

Publication

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH043

Plasmid

#186638

Purpose

aadA.co between homology arms for oscillin

Depositor

Adam Arkin

Publication

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pStA0::ECK120015170

Plasmid

#171879

Purpose

Terminator ECK120015170 in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Terminator ECK120015170

Use

Synthetic Biology

Mutation

N/A

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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(P4)TT

Plasmid

#253857

Purpose

rrnB T1 terminator derived from pTrc99A

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

rrnB T1 terminator derived from pTrc99A

Expression

Bacterial

Available since

June 5, 2026

Availability

Academic Institutions and Nonprofits only

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(P7)Pk

Plasmid

#251395

Purpose

Gene encoding pyruvate kinase (Enterococcus faecalis)

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

Pyruvate kinase

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [rev-backbone]

Plasmid

#229199

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Publication

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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scFv-SmBiT

Plasmid

#192541

Purpose

Expresses sender cell components of LOTIIS

Depositor

Jennifer Prescher

Publication

Ng et al Bioconjug Chem. 2022 Oct 19;33(10):1876-

Insert

scFv - SmBiT86

Expression

Mammalian

Promoter

CMV

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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2x GCN4-LgBiT

Plasmid

#192542

Purpose

Expresses receiver cell components of LOTIIS

Depositor

Jennifer Prescher

Publication

Ng et al Bioconjug Chem. 2022 Oct 19;33(10):1876-

Insert

2x GCN4-LgBiT

Expression

Mammalian

Promoter

CMV

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Publication

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Expression

Bacterial

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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