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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 5221 - 5240 of 9957 results

  2. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

    Plasmid
    #229223
    Purpose
    For expression of T. elongatus KaiB protein mutant
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Mutation
    1-94 (truncated), Y8A, Y94A
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229223-plasmid-map-sequence-id-457046

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  5. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  6. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  7. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  10. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Publication
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

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  11. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175788
    Purpose
    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Publication
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175788-plasmid-map-sequence-id-345285

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  14. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid
    #153445
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Publication
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153445-plasmid-map-sequence-id-306484

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  15. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Publication
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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  17. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid
    #117005
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Publication
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117005-plasmid-map-sequence-id-227039

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Showing: 5221 - 5240 of 9957 results