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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSC301b

Plasmid

#31852

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (including ATG start codon)

Expression

Bacterial

Promoter

SOD and none

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 B

Plasmid

#11325

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation B from figure 4A of paper.

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pGL2313

Plasmid

#137053

Purpose

Endogenous Locus DiCre Expression Cassette

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Type

Empty backbone

Use

Donor vector for endogenous locus dicre

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGC94

Plasmid

#19610

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.2::FLP::let-858(3’)

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR.CMV.ON

Plasmid

#32687

Purpose

Cre/lox mammalian expression and gateway entry vector

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

Cre/Lox CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSe_37

Plasmid

#109237

Purpose

PTac driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PTac

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_31

Plasmid

#109235

Purpose

PUPsp1 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PUPsp1

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJT118

Plasmid

#31395

Depositor

Christopher Voigt

Article

Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

Insert

pJT118

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pMLS272

Plasmid

#73728

Purpose

SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded C-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-sspBmyc

Plasmid

#27375

Depositor

Dirk Schnappinger

Article

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

sspBmyc

Use

Gateway destination vector

Expression

Bacterial

Mutation

N/A

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pQM

Plasmid

#48097

Purpose

Cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and myc

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMLS281

Plasmid

#73732

Purpose

SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded N-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGC95

Plasmid

#19611

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.41::FLP::let-858(3’)

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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lvl0 mTFP1 CTAG

Plasmid

#153400

Purpose

Cyan fluorescent protein with FLAG tag

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

mTFP1 CTAG

Tags

FLAG tag

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHM4 (U6 in pSE360)

Plasmid

#111281

Purpose

U6 snRNA coding sequence in pSE360(Ura) plasmid

Depositor

Christine Guthrie

Article

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

SNR6

Expression

Yeast

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pC-0_v6

Plasmid

#124426

Purpose

Full MCS, lacks araC & P(BAD) (step 2)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGC76

Plasmid

#19641

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<4xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pIB1

Plasmid

#25450

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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Auxiliary A

Plasmid

#153352

Purpose

Provides End-to-End linker 4A for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4A

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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