Skip to main content
Addgene
Showing: 7951 - 7980 of 14826 results

  1. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

    Enlarge

  2. pOCC231

    Plasmid
    #118885
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and mCitrine…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118885-plasmid-map-sequence-id-232309

    Enlarge

  6. pOPTXGARE

    Plasmid
    #68542
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…
    Promoter
    OPTX promoter with GARE
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68542-plasmid-map-sequence-id-121994

    Enlarge

  16. pcDNA3-EngPD

    Plasmid
    #64039
    Purpose
    Mammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    Pax8 paired domain (Pax8 Mouse)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    paired domain, aa 1-147
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63785-plasmid-map-sequence-id-106471

    Enlarge

  23. PatoG-OG241

    Plasmid
    #72839
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoG
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72839-plasmid-map-sequence-id-133603

    Enlarge

Showing: 7951 - 7980 of 14826 results