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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 WT

Plasmid

#199573

Purpose

Expresses FLAG-tagged WT SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Tags

histag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-NPM WT

Plasmid

#17578

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

EGFP and Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R

Plasmid

#58978

Purpose

expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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FLT3-ITD-MSCV-IRES-GFP

Plasmid

#184779

Purpose

Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

Depositor

Timothy Ley

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Activating internal tandem duplication (ITD) adde…

Promoter

MSCV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_25bpLinker

Plasmid

#157788

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_25bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

Plasmid

#246754

Purpose

RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

Use

Synthetic Biology

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTrc-trGPPS(CO)-LS

Plasmid

#50603

Purpose

pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

Depositor

Jay Keasling

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Inserts

Limonene Synthase
ApR
LacIq

Use

Synthetic Biology

Expression

Bacterial

Mutation

truncated codon optimized geranylpyrophosphate sy…

Promoter

Trc

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAXY0001

Plasmid

#179834

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Expression

Plant

Promoter

35S

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

Plasmid

#246755

Purpose

RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

Use

Synthetic Biology

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-SPI1

Plasmid

#162824

Purpose

Doxycycline-inducible overexpression of human SPI1

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet ON

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Article

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-FRT-KanR-FRT pA

Plasmid

#82601

Purpose

3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRT-Kan-FRT

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Neo

Plasmid

#125593

Purpose

Lentiviral expression of sgRNA with GFP and neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_785

Plasmid

#125852

Purpose

Expresses SUN1_785 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_785 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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