We narrowed to 15,186 results for: pET

1. pSG086

   Plasmid

   #214263

   Purpose

   PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
   

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   substitution of 31 amino acids of FtsH2 (F124RRFA…

   Promoter

   T7 promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACB120

   Plasmid

   #222213

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(K10N, S11R, W15C, G76V, G258D)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Contains five amino acid mutations: K10N, S11R, W…

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACB143

   Plasmid

   #222214

   Purpose

   For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   vanB(A24P)
   

   Tags

   N-terminal, thrombin-cleavable 6x His tag

   Expression

   Bacterial

   Mutation

   Changed Alanine 24 to Proline

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACB111

   Plasmid

   #222210

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(G76V)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed glycine 76 to valine

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACB112

   Plasmid

   #222211

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(S11R)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Serine 11 to Arginine

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACB113

   Plasmid

   #222212

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(W15C)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Tryptophan 15 to Cysteine

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FENEF-Sensor

   Plasmid

   #208930

   Purpose

   in vitro p38-Sensor for luminescent detection of p38-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   FENEF sequence from human ATF2 with phospho site (ATF2 Human)
   

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-CEP55(160-217, S215C)

   Plasmid

   #186793

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CEP55(160-217, S215C) (CEP55 Human)
   

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   S215C

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)

   Plasmid

   #199242

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
   

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   S191C, I225A, L228A, W231A

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB1-ΔN-CHMP4B(S184C)

    Plasmid

    #180026

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
    

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    S184C

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN-A112C-1A32

    Plasmid

    #191241

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN-A112C-1A31

    Plasmid

    #191242

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN-A112C-CP8B1

    Plasmid

    #191240

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ppil2.280.457.Y389H

    Plasmid

    #137658

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Tara Davis, Melissa Jurica

    Article

    Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

    Insert

    ppil2 (PPIL2 Human)
    

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    280-457-Y389H

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ppil2.280.457.Y389W

    Plasmid

    #137657

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Tara Davis, Melissa Jurica

    Article

    Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

    Insert

    ppil2 (PPIL2 Human)
    

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    280-457-Y389W

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HIS_TEV_hsSYX3

    Plasmid

    #179290

    Purpose

    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Tags

    His6 + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7lac

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GB1-PRD(703-868, P801G, T805C, C813S)-Strep

    Plasmid

    #169749

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine, Threonine 805 to Cysteine…

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GB1-PRD(703-868, P801G, G808C, C813S)-Strep

    Plasmid

    #169750

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine, Glycine 808 to Cysteine, …

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GB1-PRD(703-868, S712C, P801G, C813S)-Strep

    Plasmid

    #164445

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Serine 712 to Cysteine, Proline 801 to Glycine, C…

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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