Skip to main content
Addgene
Showing: 8011 - 8040 of 14826 results
  1. pCAGEN_MyrPalm_NES_V5_eMagAF_C74

    Plasmid
    #235639
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane
    Depositor
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    MyrPalm-NES-V5-eMagAF
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pCAGEN_V5_eMagAF_C74_cMycNLS

    Plasmid
    #235640
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus
    Depositor
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    V5-eMagAF and cMycNLS
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pCAGEN_NES_V5_eMagAF_C74_Sec61b

    Plasmid
    #235637
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum
    Depositor
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    NES-V5-eMagAF and Sec61b
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pCAGEN_NES_V5_GFP_FTA

    Plasmid
    #235633
    Purpose
    Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.
    Depositor
    Insert
    GFP
    Use
    Tags
    FLAG-TEV cleavage site-AviTag and NES-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pCAGEN_NES_V5_eMagBF_N73

    Plasmid
    #235635
    Purpose
    Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)
    Depositor
    Insert
    ALIBi N-terminal fragment
    Use
    Tags
    NES-V5-eMagBF
    Expression
    Mammalian
    Mutation
    residues 1-73
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. GB4635

    Plasmid
    #215263
    Purpose
    Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19
    Depositor
    Insert
    CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. SpCas9-NRCH

    Plasmid
    #198542
    Purpose
    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    SpCas9-NRCH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pYFAC-CH2-ubi-Y-pyrG

    Plasmid
    #221131
    Purpose
    Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pYFAC-CH2-ubi-M-pyrG

    Plasmid
    #221132
    Purpose
    Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. GB4636

    Plasmid
    #215264
    Purpose
    Module for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19
    Depositor
    Insert
    Etr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8011 - 8040 of 14826 results