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human p53-Δ(325-355)

Plasmid

#24871

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Deletion of aa 325-355

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-puro HA-WIPI-1

Plasmid

#38273

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pLVX-SV40-Galpha-q ONE-GO

Plasmid

#204331

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-q ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJAT57

Plasmid

#204303

Purpose

Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL

Plasmid

#47081

Depositor

Marina Ramirez-Alvarado

Article

McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

Insert

AL-09 (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaCC2

Plasmid

#124957

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBFP (GB0025)

Plasmid

#68193

Purpose

Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

BFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt2

Plasmid

#35906

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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IF101: pMVP (L1-L4) EF1a promoter

Plasmid

#121687

Purpose

pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE:shRNA A-pGK:rtTA-Cre

Plasmid

#68471

Purpose

Inducible lentivirus expressing an arbitrary shRNA and Cre

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

GFP-shRNA A-miR30
rtTA-2A-Cre

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

pGK and pTRE

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)

Plasmid

#192122

Purpose

Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Drosha1

Plasmid

#14780

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
HB401: pMVP (L1-L4) CMV promoter

Plasmid

#121686

Purpose

pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-oA ONE-GO

Plasmid

#204330

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-oA ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Promoter

U6

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMSC236

Plasmid

#216368

Purpose

xhNup155-Nb2i (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC235

Plasmid

#216372

Purpose

xhNup155-Nb3i (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC227

Plasmid

#216351

Purpose

xhNup35-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pETx-SR6c3

Plasmid

#176211

Purpose

Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6c3

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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