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YIp128-ADH-CUbo-VSV

Plasmid

#212732

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-VSV

Plasmid

#212730

Purpose

Copper-inducible expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-VSV

Plasmid

#212729

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-VSV

Plasmid

#212728

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-FLAG

Plasmid

#212802

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

FLAG and NUbo

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-3xFLAG-Ubc7

Plasmid

#212801

Purpose

Constitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1)-CUbo

Plasmid

#212799

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Ubc13

Plasmid

#212806

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Mms2

Plasmid

#212807

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K63R)-MCS

Plasmid

#212727

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K48R)-MCS

Plasmid

#212726

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K63R)-GFP

Plasmid

#212722

Purpose

Bacterial expression of His6-CUbo(K63R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K63R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K48R)-GFP

Plasmid

#212721

Purpose

Bacterial expression of His6-CUbo(K48R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K48R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo-GFP

Plasmid

#212720

Purpose

Bacterial expression of His6-CUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His7-NUbo-GFP

Plasmid

#212719

Purpose

Bacterial expression of His7-NUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

7xHis-NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-GFP-His6

Plasmid

#212718

Purpose

Bacterial expression of NUbo-GFP-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-His6-TEV-Mms2

Plasmid

#212716

Purpose

Bacterial expression of His6-Mms2, His6-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-TEV2-Ubc7-His6

Plasmid

#212713

Purpose

Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

6xHis and MBP-TEV2

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:TDimer mVenus

Plasmid

#210520

Purpose

Expresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Tandem dimer mVenus

Use

Zebrafish expression, tol2 kit gateway-compatible

Promoter

Zebrafish insulin promoter

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204797

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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