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AiP11415 - pAAV-AiE0007m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1415)

Plasmid

#230095

Purpose

AiE0007m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14803 - pAAV-AiE0078h_3xC2-minBG-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN4803)

Plasmid

#229927

Purpose

AiE0078h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2-10aa-H2B

Use

AAV

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13306 - pAAV-AiE0970m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3306)

Plasmid

#229911

Purpose

AiE0970m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14536 - pAAV-AiE0324m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4536)

Plasmid

#229924

Purpose

AiE0324m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15028 - pAAV-AiE1404m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5028)

Plasmid

#229929

Purpose

AiE1404m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14981 - pAAV-AiE1391h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4981)

Plasmid

#229928

Purpose

AiE1391h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14760 - pAAV-AiE2135m-minBG-iCre-BGHpA (Alias: CN4760)

Plasmid

#229926

Purpose

AiE2135m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11518 - pAAV-hsA2-AiE0017h_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1518)

Plasmid

#229881

Purpose

AiE0017h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13980 - pAAV-AiE0078h-minBG-iCre(R297T)-BGHpA (Alias: CN3980)

Plasmid

#229918

Purpose

AiE0078h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14186 - pAAV-AiE2004m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4186)

Plasmid

#229919

Purpose

AiE2004m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12042 - pAAV-3xSP10ins-AiE0355m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2042)

Plasmid

#223972

Purpose

AiE0355m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUCM-CLYBL-NGN2-BRN3A

Plasmid

#165597

Purpose

Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

Depositor

Carsten Bönnemann

Article

Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

Inserts

Neurogenin 2 (NEUROG2 Human)
BRN3A (POU4F1 Human)
EGFP
Puromycin N-acetyl transferase
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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PZ055:mOrange2-TM

Plasmid

#186430

Purpose

mOrange2-TM

Depositor

Adam Cohen

Article

Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

Insert

mOrange2-TM

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pC.HA

Plasmid

#15126

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
ZS007:eGFP-TM

Plasmid

#186429

Purpose

eGFP-TM

Depositor

Adam Cohen

Article

Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

Insert

eGFP-TM

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-PS

Plasmid

#86628

Purpose

Expresses Akt pseudosubstrate tagged with mCherry

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt pseudosubstrate (VELDPEFEPRARERAYAFGH)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEPIP_PepTile_40merFragments_65CancerDrivers

Pooled Library

#172109

Purpose

Lentiviral peptide expression library for overexpression fitness screening

Depositor

Prashant Mali

Article

Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

Use

Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only
pHaloTag-EGFP

Plasmid

#86629

Purpose

Expresses HaloTag-eGFP tandem (cytosolic)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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