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pGEX chimerin-SH2

Plasmid

#46415

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin (CHN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 44-147

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Dapp1-SH2

Plasmid

#46422

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Dapp1 (DAPP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 25-151

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16l (B694)

Plasmid

#92030

Purpose

expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16

Expression

Mammalian

Mutation

NLS sequence of CRY2 mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS-VP16

Plasmid

#103822

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-CCNA2 LONG 3'UTR

Plasmid

#29472

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CCNA2 LONG 3'UTR (CCNA2 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCIN4-EZOD

Plasmid

#44977

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 DBD (Ets2 Mouse)

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

Ets2 DNA binding domain (AA 332-468) only = domin…

Promoter

CMV

Available Since

May 28, 2013

Availability

Academic Institutions and Nonprofits only
integrin alpha9 W999A pcDNA1 neo

Plasmid

#13589

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 W999A (ITGA9 Human)

Expression

Mammalian

Mutation

W999A

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5R 99xGly-uFMR1-3xHA

Plasmid

#242192

Purpose

Transient expression of 99xGly-uFMR1-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

99xGly-uFMR1-3xHA (FMR1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2026

Availability

Academic Institutions and Nonprofits only

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pGA1-EF1α-sfGFP150TAG-4xtRNA

Plasmid

#251517

Purpose

Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

sfGFP150TAG

Tags

His6 and V5

Expression

Mammalian

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

EF1alpha

Available Since

March 12, 2026

Availability

Academic Institutions and Nonprofits only

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pT7II-2N4Rtau C291A/C322A

Plasmid

#249556

Purpose

Recombinant protein synthesis of C291A/C322A mutant of human 2N4R Tau

Depositor

Jeff Kuret

Article

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (2N4R) (MAPT Human)

Expression

Bacterial

Mutation

Cysteine 291 changed to Alanine, Cysteine 322 cha…

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_blast_FAM136A_sg2

Plasmid

#244876

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_puro_FAM136A_sg1

Plasmid

#244877

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgOMA1 sg2

Plasmid

#244866

Purpose

Knockout of human OMA1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

OMA1 sgRNA (OMA1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR_blast_FAM136A_sg1

Plasmid

#244875

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR_puro_FAM136A_sg2

Plasmid

#244878

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 sgOMA1 sg1

Plasmid

#244865

Purpose

Knockout of human OMA1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

OMA1 sgRNA (OMA1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge