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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,267 results for: trac

Showing: 5381 - 5400 of 7267 results

  1. pMTG8-Amp-FRT

    Plasmid
    #128277
    Purpose
    TIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFP
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527
    Inserts
    TetR-LOV-TIP
    EGFP
    KRAS(G12V)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128277-plasmid-map-sequence-id-251922

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  5. pcDNA5/FRT/TO/SH/GW-Mst1

    Plasmid
    #84299
    Purpose
    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.
    Depositor
    Jonathan Chernoff
    Article
    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71
    Insert
    Mst1 (STK4 Human)
    Use
    Tags
    HA, SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  9. Myc-PCDHGC5

    Plasmid
    #119935
    Purpose
    Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)
    Depositor
    Angel de Blas
    Article
    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.
    Insert
    PCDHGC5 (Pcdhgc5 Rat)
    Use
    Tags
    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    119935-plasmid-map-sequence-id-237614

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  12. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid
    #128256
    Purpose
    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.
    Depositor
    Gabor Balazsi
    Article
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Insert
    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128256-plasmid-map-sequence-id-248948

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  13. pMflPT-o4

    Plasmid
    #101315
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101315-plasmid-map-sequence-id-198861

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  14. pMflST-o4

    Plasmid
    #101316
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101316-plasmid-map-sequence-id-199230

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  18. pCIFR-5

    Plasmid
    #233294
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    p14G-BCD2
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233294-plasmid-map-sequence-id-468478

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  19. pFNC-5

    Plasmid
    #233297
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PEM7 and unknown
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233297-plasmid-map-sequence-id-468477

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Showing: 5381 - 5400 of 7267 results