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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,285 results for: crispr

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Showing: 5401 - 5420 of 25285 results

  2. pNHEJ-Cas12a

    Plasmid
    #158713
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum
    Depositor
    Yi-Cheng Sun
    Publication
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158713-plasmid-map-sequence-id-320845

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  4. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid
    #115487
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115487-plasmid-map-sequence-id-240326

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  5. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  6. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE3GS
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    136463-plasmid-map-sequence-id-272540

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  8. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid
    #196724
    Purpose
    Sortable and selectable EnAsCas12a.
    Depositor
    Bernhard Schmierer
    Insert
    enASCas12a-HA-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS - HA and SV40 NLS
    Mutation
    E174R, S542R, K548R
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196724-plasmid-map-sequence-id-388065

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  10. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid
    #98041
    Purpose
    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9-NLS-3xFLAG
    Use
    CRISPR
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    D10A + H557A
    Promoter
    CMV
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98041-plasmid-map-sequence-id-398173

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  13. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  15. pCR1054

    Plasmid
    #71822
    Purpose
    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71822-plasmid-map-sequence-id-131019

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Showing: 5401 - 5420 of 25285 results